不同烷基醛类对脱细胞-核DNA影响的研究  

Effect of different alkyl aldehydes on acellular-nuclear DNA

作　　者：李谦[1,2] 苏卿[2,3] 张文众[2] 贾旭东[2] 李宁 张立实[1] 

机构地区：[1]四川大学华西公共卫生学院,四川成都610041 [2]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021 [3]西南大学园艺园林学院,重庆400715

出　　处：《中国食品卫生杂志》2014年第6期524-527,共4页Chinese Journal of Food Hygiene

基　　金：国家自然科学基金项目(81373009);"十二五"国家科技支撑重大计划项目(2012BAK01B04)

摘　　要：目的研究甲醛、乙醛、丙醛、丁醛、己醛、戊醛对脱细胞-核DNA的影响。方法将甲醛、乙醛、丙醛、丁醛、己醛、戊醛用缓冲液分别配制浓度为10 mmol/L的溶液作为受试物;用羟自由基损伤的脱细胞-核DNA断片模型检测受试物与DNA交互作用的能力;用未经羟自由基处理的脱细胞-核DNA模型检测受试物导致DNA断裂的程度。两种模型均为每组6个平行板,处理时间均为60 min,用彗星实验检测DNA拖尾情况,每张板用彗星分析软件(Comet A1.0)分析50个脱细胞-核DNA,组间差异用SPSS 18.0软件进行统计学分析。结果经甲醛处理的脱细胞-核DNA断片模型无DNA片段泳出;经乙醛处理的脱细胞-核DNA断片模型中DNA片段泳出量明显低于对照组;其他醛类对脱细胞-核DNA断片模型的DNA断片泳出量与对照组比较差异无统计学意义;所有醛类均未导致未经羟自由基处理的脱细胞-核DNA模型的DNA断裂。结论甲醛和乙醛均可直接与脱细胞-核DNA断片形成加合物和/或产生DNA-DNA交联,且形成加合物和/或DNA-DNA交联的能力随醛类烷基数量的增加而减弱。Objective To explore the impact of formaldehyde,acetaldehyde,propionaldehyde,butyraldehyde,hexanal,and pentanal on acellular-nuclear DNA. Methods Different alkyl aldehydes were respectively dissolved in buffer to finalconcentration of 10 mmol / L. Fragment acellular-nuclear DNA pretreated with the hydroxyl radical was used to detect the interaction capabilities of test substance and DNA,intact acellular-nuclear DNA was used to detect breakage effect of test substance. Six slides with acellular-nuclear DNA,in each group,were immersed in test substances for 60 min,and comet assay was used to measure the changes of the acellular DNA,and 50 acellular-nuclear DNAs were analyzed with Comet A1. 0 software in each slide. SPSS 18. 0 software was used to compare the difference among groups. Results No fragment of acellular-nuclear DNA treated with formaldehyde migrated to tail,and fragment migration of acellular-nuclear DNA treated with acetaldehyde was more than control. All aldehydes showed no breakage on intact acellular-nuclear DNA. Conclusion Formaldehyde and acetaldehyde could form adducts and / or cross-linked with fragment of acellular-nuclear DNA. The capacity of forming adducts and or cross-linked with fragment of acellular-nuclear DNA decreased with the alkyl number of aldehyde increasing.

关 键 词：脱细胞DNA 彗星试验 醛类 DNA加合物 

分 类 号：R114[医药卫生—卫生毒理学]