新琼寡糖对保加利亚乳杆菌的促生长作用研究  

Growth-promoting effects of neoagaro-oligosaccharides on Lactobacillus delbrueckii subsp. Bulgaricus

作　　者：陈虹[1] 梅建凤[2] 应国清[2] 

机构地区：[1]浙江树人大学生物与环境工程学院,杭州310015 [2]浙江工业大学药学院,杭州310032

出　　处：《食品科技》2014年第12期24-27,共4页Food Science and Technology

基　　金：浙江省科技计划项目(2009C14027)

摘　　要：用来源于类芽孢杆菌(Paenibacillus sp.)的β-琼胶酶水解琼胶制备寡糖,经过活性炭脱盐和去杂后,寡糖的主要组分为新琼四糖和新琼六糖。新琼寡糖对保加利亚乳杆菌(Lactobacillus delbrueckii subsp.Bulgaricus)的生长有显著的促进作用,MRS培养基中添加浓度为10 g/L的新琼寡糖,与对照相比,保加利亚乳杆菌的生长量提高了77.6%,即使新琼寡糖浓度高达100 g/L时,也不会对保加利亚乳杆菌的生长产生抑制;体外实验表明,新琼寡糖不会被人体唾液淀粉酶消化。新琼寡糖的这些特性表明其具有作为益生元在食品工业中的应用价值。Neoagaro-oligosaccharides（NAOS） were prepared from agar hydrolysis by a β-agarase, which was secreted by Paenibacillus sp. The main components of the hydrolyzates were neoagarotetraose and neoagarohexaose after desalination and edulcoration by activated carbon adsorption. The results showed that the NAOS could significantly promote the growth of Lactobacillus delbrueckii subsp. Bulgaricus. When 10 g/L of NAOS was supplied into Man-Rogosa-Sharp（MRS） medium, the growth of L. Bulgaria was promoted by 77.6% compared with the control. Even the concentration of NAOS was up to 100 g/L in MRS, no growth inhibition of L. Bulgaria was observed. In vitro, NAOS could not be digested by salivary amylase. These characteristics of NAOS indicated that it can be developed as a prebiotic additive for the food industry.

关 键 词：Β-琼胶酶 新琼寡糖 益生元 保加利亚乳杆菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]