动物双歧RH发酵雪莲果胡萝卜复合饮料的研制  

Manufacture of fermented smallanthus sonchifolius and carrots juice with Bifidobacterium animalis RH

作　　者：韦云路 张丽末 唐虞木子 郭秀锋[1] 王洋[1] 李平兰[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,教育部-北京市共建功能乳品重点实验室,北京100083

出　　处：《食品科技》2014年第12期121-127,共7页Food Science and Technology

基　　金：国家自然基金项目(31271827);2014年北京市大学生创新训练项目(2014bj061)

摘　　要：为制备活菌型双歧杆菌合生元饮品,以富含双歧因子的胡萝卜、雪莲果为主要原料,采用动物双歧杆菌RH进行发酵。动物双歧RH来源于中国长寿老人肠道,可产活性多糖。通过Plackett-Burman从多种碳源、氮源及生长因子中筛选对活菌数贡献较大的因素,进一步通过最陡爬坡试验确定各因素的水平。最后利用旋转中心组合试验(Central Composite Design)得到因素水平与活菌数方程,并经验证试验得到最佳果汁配方为:雪莲果汁体积分数20.00%、果糖添加量0.50 g/L、脱脂乳粉添加量13.88 g/L、番茄汁添加量8.28%。最佳配方条件下发酵果汁中双歧杆菌的活菌数可以达到3.35×108 cfu/mL,多糖含量为1220 mg/L。Fermented juice was manufactured with smallanthus sonchifolius（yacon） and carrots by Bifi dobacterium animalis RH. B.animalis RH with exopolysaccharides producing ability were derived from centenarians faeces. Factors signifi cantly affecting the viable count of bifidobacteria were selected from a variety of carbon, nitrogen sources and bifi dus factors by Plackett-Burman test. And the level of each factor were determined subsequently by steepest ascent test. The formulars of the fermented juice were finally optimized with a central composite design involving the above 4 factors at 5 levels. The results showed that the optimum juice formula is： smallanthus sonchifolius juice 20.00%, fructose content 0.50 g/L, skim milk 13.88 g/L and tomato juice 8.28%. In the optimal formula, the viable count of Bifidobacterium could reach up to 3.35×10^8 cfu/m L, and content of polysaccharide was 1220 mg/L.

关 键 词：双歧杆菌 合生元 雪莲果 胡萝卜 胞外多糖 

分 类 号：TS275.5[轻工技术与工程—农产品加工及贮藏工程]