胶束电动毛细管色谱法分析辣椒制品中5种苏丹红  

Micellar electrokinetic capillary chromatography for simultaneous determination of five Sudan dyes in chili products

作　　者：胡江涛[1] 张伟[2] 陈璐莹[2] 孙成均[2,3] 

机构地区：[1]四川省出入境检验检疫局,成都610041 [2]四川大学华西公共卫生学院,成都610041 [3]四川省食品安全监测与风险评估重点实验室,成都610041

出　　处：《分析试验室》2014年第12期1408-1412,共5页Chinese Journal of Analysis Laboratory

基　　金：四川省科技支撑计划(2012FZ0088)资助

摘　　要：建立了同时测定辣椒粉、辣椒油和辣椒酱中苏丹红Ⅰ,Ⅱ,Ⅲ,Ⅳ,G的胶束电动毛细管电泳分析方法,样品经正己烷提取、中性氧化铝柱净化、再经丙酮-正己烷（5∶95,V/V）洗脱,经氮气吹干、乙腈溶解,用40 mmol/L硼砂-40 mmol/L SDS按1∶2的比例稀释后进样。运行缓冲液为2.5 mmol/L硼砂-30 mmol/L SDS-40%乙腈（p H 9.2）。5种色素在13 min内达到基线分离,标准曲线线性范围为1-20 mg/L,线性关系良好（r〉0.997）,检出限范围为0.12-0.62μg/m L,定量限范围为2.4-12.4 mg/kg。加标回收率为76.3%-98.6%,相对标准偏差（RSD）小于5%。A method for simultaneous determination of Sudan red I - IV and G in chili products including chili powder,chili oil and chili sauce by micellar electrokinetic capillary chromatography was established. The samples were extracted with n-hexane,purified and enriched with neutral alumina column. The target dyes were eluted with 5% acetone-n-hexane,and dried-up under nitrogen flow. The residue was redissolved in acetonitrile and then diluted with 40 mmol / L borax- 40 mmol / L SDS（ 1： 2,V / V） for analysis. The target dyes were separated with a capillary with the running buffer consisted of borax,sodium lauryl sulfate and acetonitrile（ p H9. 2）. Under the optimized conditions,the five azo-dyes were baseline separated within 20 min. The linear ranges of the method were 1 - 20 mg / L and the correlation coefficients were greater than 0. 997. The detection limits were 0. 12 - 0. 62 μg / m L and the quantification limits were 2. 4 - 12. 4 mg / kg. The recoveries were76. 3% - 98. 6% and the relative standard deviations（ RSDs） were less than 5%.

关 键 词：胶束电动毛细管色谱法 苏丹红 辣椒制品 

分 类 号：O657.8[理学—分析化学]