长托宁对热性惊厥幼鼠海马神经元的保护作用  

Neuroprotective Effect of Penehyclidine Hydrochloride in the Damage of Hippocampal Neuron Caused by Febrile Seizure

作　　者：邵巧燕[1] 戴锦泉 吴斌[1] 施晓容[1] 陈林莺[3] 陈余朋[3] 

机构地区：[1]福建医科大学附属第一医院小儿科,福州350005 [2]福建省福州儿童医院儿科,福州350005 [3]福建医科大学附属第一医院病理科,福州350005

出　　处：《福建医科大学学报》2014年第4期232-236,共5页Journal of Fujian Medical University

摘　　要：目的探讨长托宁(盐酸戊乙奎醚注射液)干预对热性惊厥(FS)幼鼠海马神经元损伤的保护作用及其可能机制。方法 54只14日龄新生SD幼鼠随机分为3组,即盐水对照组、FS模型组、长托宁干预组,根据处死时间再分为12h、24h及48h亚组。采用低剂量海人藻酸与脂多糖腹腔内注射建立幼鼠FS模型,长托宁干预组在建模同时给予长托宁0.45mg/kg,观察比较各组幼鼠惊厥表现,采用苏木精-伊红(H-E)染色法观察记录各组幼鼠海马CA1区神经元结构变化,应用原位末端标记法(TUNEL)观察并记录各组海马神经细胞凋亡数。结果 LPS联合低剂量KA诱导FS模型组与长托宁干预组幼鼠惊厥发作,建立FS幼鼠模型。FS模型组、长托宁干预组幼鼠惊厥潜伏期差别无显著性,惊厥持续时间分别为(27.74±6.92)min和(21.74±6.60)min,惊厥程度评分(级)分别为(3.80±0.94)min和(3.18±0.92)min,差别均有统计学意义(P均<0.05)。与FS模型组比较,长托宁干预组各时间点海马CA1区神经元变性及丢失程度减轻。与FS模型组比较,长托宁干预组各时间点海马CA1区TUNEL阳性细胞数均明显减少(P<0.05)。结论长托宁通过减少海马部位神经元细胞凋亡对FS幼鼠海马神经元有保护作用。Objective The main purpose of the present study was to find out whether the penehy-clidine hydrochloride （PHC） has the neuroprotective effect on seizure-induced hippocampal neuron dam-age . Methods Sixty-four cases of 14-day-old Sprague-Dawley rats were divided into three groups ran-domly ：the normal saline control group ,the hyperthermic seizure group ,and the PHC treated group . In-traperitoneal injection of LPS combined with KA was used to establish rat model with FS . The rats were injected intraperitoneally with PHC at each time （15 minutes before modeled ,every 12 hour after mod-eled） . Then onset latency ,duration and grade of FS in different groups were observed and compared . Then the histopathology changes in hippocampus were viewed by HE staining and electron-microscope ,the neuron apoptosis was detected by TdT-mediated dUTP nick end labeling （TUNEL） . Results LPS com-bined with KA of intraperitoneal injection can induce febrile seizure . In PHC treated group ,the rats FS duration and FS grade were significantly lower than those in FS control group （P〈0 .05） ,although no sig-nificant gap was observed on FS onset latency between them . In FS control group ,HE-staining pattern of hippocampal CA1 region showed lots of disordered neurons with confused polarity and vacuoles formed . While in PHC treated groups ,the arrangement of neurons were regular ,only a small number of neurons had changed . In FS group ,at the 24 h point after seizure ,TUNEL-positive cell in hippocampus CA1 re-gion increased most significantly comparing with PHC treated group （P〈0 .05） . Conclusion The pene-hyclidine hydrochloride can lighten the febrile seizure-induced hippocampal neuron damage in rats by inhib-iting cell apoptosis .

关 键 词：奎宁环类 海马 惊厥  发热性 脂多糖类 藻酸盐 红藻氨酸 脑损伤 细胞凋亡 疾病模型  动物 

分 类 号：R742.1[医药卫生—神经病学与精神病学]