利用枯草芽孢杆菌发酵生产环磷腺苷的工业化试验  

Process of Adenosine-3',5'-Cyclic Monophosphate Production by Fermentation with Bacillus. subtilis

作　　者：朱晓宏[1] 朱晓慧[1] 魏薇[1] 

机构地区：[1]江苏省微生物研究所有限公司,江苏无锡214063

出　　处：《食品与生物技术学报》2014年第11期1228-1231,共4页Journal of Food Science and Biotechnology

基　　金：江苏省自然基金项目(BK2009075)

摘　　要：为了实现环磷腺苷的发酵生产,对一株枯草芽孢杆菌Bacillus.subtilis jsim-1277在摇瓶、自控罐、20 M3发酵罐上的发酵条件进行了试验。结果表明,最适宜发酵条件是p H 6.7,且添加K2HPO41.0 g/d L,Na F 0.05 g/d L;在摇瓶、自控罐、大型发酵罐产苷分别为7.17、6.01、7.10 g/L。In order to prepare the adenosine-3＇,5＇-cyclic monophosphate by fenmetation,the ferment conditions of Bacillus.subtilis jsim-1277 were studied in shake flask,auto fermentor,and 20 M3 fermentor.The results showed that the optimal conditions for the fermentation were identified as follow：pH at 6.7,the optimal concentration of K2HPO4 was 1.0 g/dL,the optimal concentration of NaF was 0.05 g/dL.Under the optimized fermentation conditions,the productivity in shake flask,auto fermentor,or 20 M3 fermentor was 7.17,6.01,7.10 g/L,respectively.

关 键 词：枯草芽孢杆菌 环磷腺苷 发酵条件 

分 类 号：TQ929[轻工技术与工程—发酵工程]