麻栎树皮多酚含量的测定及抗氧化活性的研究  

Polyphenol content and antioxidant activity of the barkof Quercus acutissima Carruth

作　　者：杨春涛[1,2] 曾晓丽[1,2] 戴建辉[1,2] 孙静贤[1,2] 李世源[1,2] 李新明[1,2] 黄相中[1,2] 田凯[1,2] 

机构地区：[1]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500 [2]云南民族大学云南省生物高分子功能材料工程技术研究中心,云南昆明650500

出　　处：《云南民族大学学报（自然科学版）》2014年第6期404-407,共4页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(21262047);云南省应用基础研究项目(S2012FZ0227);云南民族大学传统傣药研究创新团队项目;SRT创新性实验项目(2013HXSSRTY06)

摘　　要：采用分光光度法、DPPH法和连苯三酚红(PR)法对麻栎树皮提取物进行多酚化合物含量及抗氧化活性测定,讨论了用不同溶剂提取对多酚含量的影响.结果表明采用70%乙醇进行提取,提取物中多酚含量最高,为(6 589.91±43.32)mg/100 g.抗氧化活性测试中,麻栎树皮的不同溶剂提取物对DPPH自由基和羟自由基都表现出较高的清除率,其中麻栎树皮70%丙酮提取物的活性最好,在0.18 mg/m L质量浓度下,对DPPH自由基清除率为70%,在0.38 mg/m L质量浓度下,对羟自由基氧化率为75%.Polyphenol content of the extracts from the bark of Quercus acutissima Carruth was determined by spectrophotometric method.The antioxidant activity of the extracts was evaluated by the DPPH free radical scavenging assay and pyrogallol autoxidation assay,respectively.Furthermore,the polyphenol contents of different extracts from this plant were investigated.The results show that the polyphenol content of 70% ethanol extract was the highest,which was（6 589.91 ± 43.32）mg/100 g.All of the different extracts from Q.acutissima have good activities against DPPH free radical and hydroxyl free radical,the results show that the 70% acetone extracts have exhibited better antioxidant activity against DPPH free radical at the concentration of 0.18 mg/mL with the inhibition rate of 70%,and 75% against hydroxyl free radical at 0.38 mg/mL.

关 键 词：麻栎 多酚类 抗氧化活性 

分 类 号：R931.6[医药卫生—生药学]