淡紫拟青霉E16液体发酵工艺研究  

Study on Liquid Fermentation Technique of Paecilomyces lilacinus E16

作　　者：张建华[1] 梁昌聪[1] 刘磊[1] 汪军[1,2] 王伟伟[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物检测与控制重点实验室,海南海口571101 [2]海南大学环境与植物保护学院,海南海口571101

出　　处：《河南农业科学》2014年第11期87-92,共6页Journal of Henan Agricultural Sciences

基　　金：公益性行业(农业)科研专项(200903049);农业科技成果转化资金项目(2012GB23260563)

摘　　要：以活菌数为检测指标,通过单因子和正交试验对淡紫拟青霉E16液体发酵工艺进行优化。结果表明,淡紫拟青霉E16发酵最佳液体培养基配方为:白砂糖1.5%(质量分数,下同),大豆粉1%,磷酸二氢钾0.1%,硫酸锌0.01%;最佳发酵条件为:起始pH值6,500 mL三角瓶装液量30%,接菌量5%,培养温度30℃,摇床转速150r/min,培养时间4d。在最佳发酵培养基和发酵条件下,淡紫拟青霉E16产生的活菌数为9.80×109 cfu/mL,较优化前提高近1个数量级。According to the number of viable cells,the liquid fermentation technique of Paecilomyces lilacinus E16 was optimized by the single factor and orthogonal design experiments.The best liquid medium of E1 6 was determined as following：sugar 1.5%（mass fraction）,soybean powder 1%,potassium dihydrogen phosphate 0.1%,zinc sulfate 0.01%.And the optimum culture conditions were determined as following：an initial pH value of 6,500 mL flasks,liquid volume of 30%,inoculum amount of 5%,culture temperature of 30 ℃,shaking speed of 150 r/min, cultivating for 4 d.Under the optimal medium and fermentation conditions,the number of viable cells of P.lilacinus E16 was 9.80×109 cfu/mL,increasing by one order of magnitude compared with before optimization.

关 键 词：淡紫拟青霉 液体发酵 培养基 发酵条件 活菌数 正交试验 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]