产D-乳酸菊糖芽孢乳杆菌的诱变及发酵条件研究  

Mutation Breeding and Optimization of Fermentation for Producing D-lactic Acid with Sporolactobacillus inulinus

作　　者：刘联杰 周安盛[1] 方聪明 王常高[1] 林建国[1] 杜馨[1] 蔡俊[1] 

机构地区：[1]湖北工业大学发酵工程教育部重点实验室/工业发酵湖北省协同创新中心,武汉430068

出　　处：《湖北农业科学》2014年第20期4936-4940,共5页Hubei Agricultural Sciences

摘　　要：以菊糖芽孢乳杆菌(Sporolactobacillus inulinus)为出发菌株,经紫外线、硫酸二乙酯的诱变处理,采用碳酸钙平板初筛及摇瓶复筛得到1株高产D-乳酸的正向突变株D11。通过单因素试验研究D11菌株发酵产D-乳酸的条件。结果表明,当发酵温度37℃,初始p H 6.5,转速160 r/min,接种量5%,装液量50m L/250 m L,发酵68 h,该菌株D-乳酸产量达到56.18 g/L,比出发菌种提高49.53%。After the original strain Sporolactiobacillus inulinus was mutated by UV and DES and selected by CaCO3 plates,a D-lactic acid yield positive mutant D1l by fermenting in shake flask was obtained.Through the single factor experiment,the fermentation conditions of producing D-lactic acid was studied.The results showed that the D-lactic acid production reached 56.18g/L when the fermentation temperature was 37 ℃,initial pH was 6.5,the rotation speed was 160 rpm/min,inoculums was 5％（V/V）,liquid volume was 50 mL/250 mL,fermentation time 68 h.The D-lactic acid production reached 56.18 g/L and was enhanced by 49.53％ compared with original strain.

关 键 词：菊糖芽孢乳杆菌(Sporolactobacillus inulinus) D-乳酸 诱变育种 正向突变 

分 类 号：Q935[生物学—微生物学]