高效液相色谱法对水产品中虾青素的测定  

Determing Astaxanthin in Aquatic Products with HPLC

作　　者：吴祥庆[1] 黎小正[1] 杨姝丽[1] 吴明媛[1] 谢宗升[1] 陈静[1] 秦振发[1] 黄鸾玉[1] 

机构地区：[1]广西水产科学研究院广西渔业病害防治环境监测和质量检验中心,南宁530021

出　　处：《湖北农业科学》2014年第20期4958-4960,共3页Hubei Agricultural Sciences

基　　金：广西区直属公益性科研院所基本科研项目(GXIF-2012-9)

摘　　要：建立水产品中虾青素含量的高效液相色谱测定方法.样品经乙腈萃取、超声提取、离心后,将上清液旋转蒸发、定容,用液相色谱-紫外检测器检测,外标法定量.虾青素浓度在0.02- 10.00 μg/mL范围内线性关系好,相关系数0.999 5;加标回收率为91.9％-95.8％,检出限为10.00 μg/kg.该方法准确度高,重现性好,易操作,适用于水产品中虾青素含量的测定.A HPLC method was developed to analyzed of astaxanthin in aquatic products.The samples were ultrasonically extracted with acetonitrile after centrifugation.The supernatant was evaporated and resolved.The determination was performed by HPLC with UV detector.The external calibration standard was used for quantification.The linear range was 0.02-10 μg/ mL （R=0.999 5）with a good linearity.The recovery range was in 91.9％-95.8％ with the detection limit of 10 μg/kg.The method is accurate,reproducible,easy for determing astaxanthin in aquatic products.

关 键 词：高效液相色谱法 虾青素 水产品 

分 类 号：O657.6[理学—分析化学]