大蒜在葱科的分子分类地位研究  

Molecular Phylogeny Analysis of Allium Sativum in Alliaceae

作　　者：侯进慧[1] 李同祥[1] 蔡文佳[1] 

机构地区：[1]徐州工程学院,江苏徐州221018

出　　处：《徐州工程学院学报（自然科学版）》2014年第4期47-50,共4页Journal of Xuzhou Institute of Technology(Natural Sciences Edition)

基　　金：江苏省高校自然科学研究项目(12KJD550003);江苏省博士后基金项目(1302041B);徐州工程学院校级科研课题(2013);徐州工程学院大学生创新项目

摘　　要：通过扩增获得18SrRNA和叶绿体16SrRNA基因序列,测序并提交GenBank,登录号分别是JF719285和JF719286.利用大蒜和GenBank相关序列构建系统发育树,进行分子演化分析.结果表明:大蒜18SrRNA基因与球序韭、韭菜、茖葱等葱科植物序列相似度高;叶绿体16S rRNA基因与龙舌兰科和薯蓣科的物种序列相似度高.大蒜与葱科植物在18SrRNA序列上具有较高的同源性.18SrRNA序列在植物演化方面的区分度比16SrRNA高.In the paper ,molecular phylogeny of Allium sativum were discussded with the analysis of rRNA gene .18S rRNA gene and chloroplast 16S rRNA gene sequences were amplified .The two rRNA genes were submitted to Genbank and the accession numbers were JF719285 and JF719286 .Gene sequences of Allium sativum was analyzed with related species in GenBank .The results showed that ：Allium sativum 18S rRNA gene has a high homology with many species within Alliaceae ,such as Allium thunbergii ,Allium tuberosum and Allium victorialis .Allium sativum and Alliaceae plants has a high similarity in 18S rDNA . The discrimination accusation of 18Sr RNA sequences in plant phylogeny analysis is better than that of 16S rRNA .

关 键 词：大蒜 RRNA 分类 鉴定 

分 类 号：Q946[生物学—植物学]