微环境中黑曲霉脂肪酶催化油脂水解性能研究  

Lipase Catalyzed Hydrolysis of Safflower Oil in Microemulsion Media

作　　者：甘争艳[1] 阿依木妮沙[1] 贾殿赠[2] 

机构地区：[1]新疆轻工职业技术学院,新疆乌鲁木齐830021 [2]新疆大学,新疆乌鲁木齐830046

出　　处：《山东化工》2014年第12期19-21,共3页Shandong Chemical Industry

摘　　要：研究了微乳液体系中黑曲霉脂肪酶LIPASE LVK催化红花油水解反应,考察了表面活性剂种类﹑微乳液ω0值、表面活性剂浓度、反应温度﹑p H值、添加剂等因素对水解率的影响,确定了微乳液体系中脂肪酶LIPASE LVK水解红花油的适宜条件:以异辛烷为有机溶剂,[AOT]=0.1mol/L,ω0=49,反应温度25℃,缓冲溶液的p H值为8.0,不加添加剂。Enzymatic hydrolysis of safflower oil catalyzed by a lipase from Aspergillus niger was investigated in a Microemulsion Media, and the effect of the surfactant, the ω0 value of the Microemulsion, the concentration of the surfaetant,temperature,pH and the additive on the hydrolysis was studied. The optimum conditions for the reaction were 25℃ ,pH =8.0, ω0 =49,[ AOT] = 0. 1mol/L, isooctane as the solvent and no additive.

关 键 词：水解 红花油 微乳液体系 脂肪酶 

分 类 号：TQ925.6[轻工技术与工程—发酵工程]