碱液浸提法制备硫酸软骨素的工艺优化  

Optimization of preparation of chondroitin sulfate by alkali leaching method

作　　者：叶琳弘 刘秋凤[2] 王茵 吴成业[2] 

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]福建省水产研究所,福建厦门361013

出　　处：《福建水产》2014年第6期428-435,共8页Journal of Fujian Fisheries

基　　金：福建省海洋经济创新发展区域示范项目(2013fj09)

摘　　要：采用碱液浸提法对鱿鱼软骨中硫酸软骨素的提取工艺进行研究。以硫酸软骨素得率为评价指标,通过对影响硫酸软骨素得率的因素进行单因素试验和响应面优化试验确定最佳工艺参数。结果表明,碱液浸提的最佳工艺条件为:料液比1∶8(g/m L)、浸提温度50℃、浸提时间5.3 h,NaOH浓度3%。在此条件下,鱿鱼软骨中硫酸软骨素得率为62.89%。The alkali leaching method was used in the extraction process of chondroitin sulfate from squid cartilage. Choosing chondroitin sulfate yield as evaluation index,the best technology parameters were confirmed by single factors and surface response optimization tests. The optimum conditions of leaching technology were obtained as follows： ratio of material to solvent was 1 ∶ 8（ g / m L）,leaching temperature was 50℃,leaching time was 5. 3 h,alkali concentration was 3%. Under these conditions,the yield of chondroitin sulfate from squid cartilage was 62. 89%.

关 键 词：鱿鱼软骨 碱液浸提 硫酸软骨素 响应面优化 

分 类 号：S937.3[农业科学—渔业资源]