鲟鱼硫酸软骨素对小鼠免疫器官肥大细胞的影响  

The effect of sturgeon chondroitin sulfate on the number of mast cells in immune organ of mice

作　　者：黄世玉[1] 李江森[1] 关瑞章[1] 

机构地区：[1]集美大学水产学院,农业部东海海水健康养殖重点实验室,福建厦门361021

出　　处：《福建水产》2014年第6期442-445,共4页Journal of Fujian Fisheries

基　　金：福建省海洋与渔业厅重点项目[闽海渔(2009)1-11号]

摘　　要：为研究鲟鱼硫酸软骨素对小鼠免疫器官的影响,连续对小鼠灌胃不同剂量鲟鱼硫酸软骨素2周后,外科手术取出胸腺和脾脏,并依次进行称重、卡洛氏固定、石蜡包埋、组织切片、甲苯胺蓝染色,40倍物镜下观察鲟鱼硫酸软骨素对小鼠免疫器官肥大细胞数量的影响。结果表明:鲟鱼硫酸软骨素能促进小鼠免疫器官重量指数增大,并能显著促进免疫器官中肥大细胞数量的增长。其中,600 mg/kg剂量下小鼠胸腺指数显著(P<0.05)增大,胸腺中肥大细胞数量极显著(P<0.01)增加,60 mg/kg剂量下小鼠脾脏重量的增加最为明显(P<0.01),6 mg/kg剂量下小鼠脾脏中肥大细胞数量的增加最为明显(P<0.01)。To study the effect of sturgeon chondroitin sulfate（ Ch S） on immune organs of mice,the mice were administered with the different doses of sturgeon chondroitin sulfate. After two weeks,the thymus and spleens were collected by surgical operation and were weighed,then were processed by being fixed in Calot＇s,paraffin embedded,made slice and modified toluidine blue（ MTB） staining. The distribution and number of mast cells of mouse were observed by looking through a microscope. The results were showed that the different doses of sturgeon chondroitin sulfate could enhance the weight of the immune organ and increase the number of mast cell in the immune organ of mice,among which,administered with the dose of 600 mg / kg could enhance significantly the weight of the thymus（ P〈0. 05） and increase the number of the mast cells in the thymus（ P〈0. 01）,and administered with the dose of 60 mg / kg could enhance significantly the weight of the spleen（ P〈0. 01）. Only administered with the dose of 6mg / kg could increase the number of the mast cells in the spleen evidently（ P〈0. 01）.

关 键 词：鲟鱼 硫酸软骨素 免疫器官 肥大细胞 

分 类 号：S985.1[农业科学—捕捞与储运]