乙烷基亚硝基脲诱变获得一例小眼畸形小鼠及其遗传实验  

Microphthalmia Mutagenesis Mouse Induced by N-ethyl-N-nitrosourea and Its Genetic Tests

作　　者：王建明[1,2] 耿滕[2] 陈航 倪俊达[2] 王文华[2] 陈兵[1,2] 薛整风[1,2] 

机构地区：[1]扬州大学比较医学中心,扬州225009 [2]扬州大学兽医学院,扬州225009 [3]扬州大学实验室与设备管理处,扬州225009

出　　处：《实验动物与比较医学》2014年第6期473-475,共3页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金项目（NO：31372269）,扬州大学科技创新培育基金项目（NO：2013CXJ064）

摘　　要：目的 通过乙烷基亚硝基脲（ENU）诱导小鼠突变手段获得眼部异常小鼠模型.方法 选用8～ 10周龄C57BL/6J （B6）雄鼠40只,腹腔内注射ENU.将处理雄鼠与同品系母鼠交配,在其后代小鼠中筛选眼部异常个体,并对突变个体进行遗传实验.结果 通过ENU诱变手段获得一例可稳定遗传的小眼畸形小鼠,该小鼠小眼畸形表型受小鼠遗传背景影响,在B6背景中外显率为90％,BDF1背景中外显率为0,BDBN2背景为27.8％.结论 ENU诱变获得了类似人类小眼畸形疾病的小鼠模型,为人类相应眼病研究提供一种新模型.Objective To obtain the eye mutation mice induced by ethylnitrosure （ENU） which would offer the models for human eye diseases research.Methods N-ethyl-N-nitrosourea （ENU） was intraperitoneally injected in forty C57BL/6J （B6） male mice aged 8-10 weeks old.The male mice were mated with the same strain female mice,and their progenies were screened for visible eye mutation.Genetic tests were carried out.Results An inherited microphthalmia mouse was obtained by ENUinduced mutagenesis.Genetic tests showed that genetic background can affect the penetrance of the defect.The penetrance of observed eye defect were 90％,0％ and 27.8％ on the B6,BDF1 and BDBN2,respectively.Conclusion The microphthalmia mice models was obtained by ENU-induced mutagenesis,it may be used for human microohthalmia disease research.

关 键 词：乙烷基亚硝基脲 小眼畸形 诱变 小鼠模型 

分 类 号：R771.1[医药卫生—眼科] R-332[医药卫生—临床医学]