乳铁蛋白检测方法研究进展  

Advances in Detection Methods for Lactoferrin

作　　者：涂茂林 杨戬[1] 刘猛[1] 石璞洁 王韫[1] 樊凤娇[1] 杜明[1] 张兰威[1] 

机构地区：[1]哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨150090

出　　处：《乳业科学与技术》2015年第1期32-37,共6页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金："十二五"国家科技支撑计划项目(2013BADl8806-03);国家自然科学基金青年科学基金项目(31101316);国家自然科学基金面上项目(31371805);教育部新世纪优秀人才计划项目(NCET一11-0796);黑龙江省博士后研究人员落户黑龙江科研启动金;中央高校基本科研业务费专项基金项目(HIT.BRETIII.201231)

摘　　要：乳铁蛋白是一种重要的多功能蛋白,具有抗菌、抗肿瘤、抗过敏、调节免疫等多种生物学活性,相关基础研究及产品开发已经引起了广泛关注。因此,乳铁蛋白检测方法也备受关注。尤其是不同方法各自的优势、灵敏度、准确度及稳定性等方面具有一定的差异,这也直接影响着该方法的适用范围。本研究对适合不同类型生物样品中乳铁蛋白的检测技术进行了阐述,主要有分光光度法、高效液相色谱法、酶联免疫吸附法、高效亲和色谱法、高效毛细血管电泳法以及表面等离子共振技术等检测技术,并分析比较了这些检测技术的不同优缺点,以期能为乳铁蛋白的研究与开发实践中选择恰当的检测方法提供一定的参考。Lactoferrin is an important multifunctional protein that has a variety of biological activities,such as anti-bacterial,anti-tumor,anti-allergy,immunoregulatory and so on.The related basic research and application development of lactoferrin have attracted wide attention.Therefore,the.detection methods for lactoferrin have drawn increasing attention.Especially the advantages,sensitivity,accuracy and stability of different methods vary to different extents,which directly affects their applicable scope.The present paper has described the detection methods which have been reported to be suitable for different types of biological samples,mainly including spectrophotometry,high performance liquid chromatography （HPLC）,enzyme-linked immunosorbent assay （ELISA）,high affinity chromatography （HPAC）,efficient capillary electrophoresis （HPCE） and surface plasmon resonance （SPR）.The advantages and disadvantages of these different detection technologies also have been analyzed and compared.This review is expected to provide reference for selection of the suitable detection method for lactoferrin in research and development practices.

关 键 词：乳铁蛋白 检测方法 研究进展 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]