LEA-motif对胰岛素在低温干燥过程中活性保护的研究  

Investigation on bioactive protection of LEA-motif to insulin during desiccation

作　　者：张燕[1] 李代禧[1] 郭柏松 翟振[1] 刘立[1] 杨春生 余华星[1] 

机构地区：[1]上海理工大学食品科学与工程研究所,上海200093 [2]上海东富龙科技股份有限公司,冻干工艺研究室,上海201108

出　　处：《食品工业科技》2015年第2期177-180,185,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(51076108);上海市国际合作项目(12430702000);上海市自然科学基金(12ZR1420400);上海市联盟计划项目及上海市重点学科项目(T0503和P0502)

摘　　要：选用LEA蛋白特征重复片段(LEA-motif)作为干燥活性保护剂,以胰岛素为研究对象,研究该天热保护剂对热敏性胰岛素的干燥保护效果和作用机理。模拟采用Gromacs软件及Gromos96(53a6)简化原子力场,并采用REMD广泛采样分子模拟方法。研究结果表明,LEA蛋白特征重复片段对胰岛素的三维活性结构、二级结构都有良好的保护作用。另外,从实验验证结果可知,有LEA-motif保护的胰岛素六聚体活性明显提高。综上可知,LEA蛋白特征重复片段对热敏性蛋白药物有良好的干燥活性保护作用。In the paper,insulin was taken as the model of heat-sensitive drugs,and LEA-motif as the bioactive protectant. Gromacs software with Gromos96（ 53a6） forcefield was adopted to investigate the LEA- motif protective effects on insulin by REMD molecular simulation method. Results showed a significant protective effect on 3D structure and secondary structure of insulin with the presence of LEA- motif. In addition, the potency experiment by HPLC showed the bioactivity of the rehydrated insulin with LEA-motif was higher than that of the rehydrated insulin without any protectant. In a word,LEA-motif had shown its great application value as a drying protectant for heat-sensitive protein drugs.

关 键 词：胰岛素 LEA-motif 分子模拟 干燥活性保护 结构稳定性 效价 

分 类 号：TS201.2[轻工技术与工程—食品科学]