不同地域野生欧李及其近缘植物亲缘关系的RAPD分析  

Genetic Diversity among Wild Chinese Dwarf Cherry from Different Regions and its Closely Related Plant Species Based on RAPD Analysis

作　　者：王鹏飞[1] 李利锋 杜俊杰[1] 张建成[1] 纪薇[1] 穆霄鹏[1] 曹琴[1] 

机构地区：[1]山西农业大学园艺学院,太谷030801 [2]山西国瑞药业有限公司,太原030500

出　　处：《植物遗传资源学报》2015年第1期119-126,共8页Journal of Plant Genetic Resources

基　　金：教育部高等学校博士学科点专项科研基金(20121403110003);山西省科技基础条件平台建设项目(2013091004-0101);山西省科技重大专项(20121101010);山西农业大学育种基金(2006060)

摘　　要：利用RAPD分子标记技术对21个不同地域欧李及其近缘种属植物(桃、杏、李、樱桃)进行了遗传亲缘关系及分类研究。结果表明,用于不同地域野生欧李分析的25条引物中,共扩增出441个清晰可用的DNA片段,其中多态性位点366个,多态性比为82.99%。在遗传相似系数为0.71时,可将中国野生欧李资源划分为3个大的区域,分别为东北分布群、华北华东分布群与中西部分布群。用于欧李及其近缘种属植物分析的20条引物中,共扩出多态性带683条,多态性比率为98.99%。聚类分析表明,在遗传相似系数为0.61时,欧李与其近缘种属植物分为3大类;欧李与麦李的亲缘关系最近,与桃亲缘关系最远。Random amplified polymorphic DNA（RAPD） was used to evaluate the genetic relationship among 21 Chinese dwarf cherry wild resources accessions from different regions and four close relatives （ peach, apricot, plum and cherry etc. ）. The results of UPGMA cluster proved that 25 primers were selected for amplification wild Chinese dwarf cherry from different regions of unambiguous and high polymorphic bands. These 25 primers amplified total 441 bands out of which 366 bands（82.99% ）were polymorphic. UPGMA cluster analysis of the similarity matrix ob- tained from the RAPD database resulted in a dendrogram of genetic relationships that grouped 21 accessions into 3 clusters at the genetic similarity value of 0.71. They were Northeast distribution group,Northern China and eastern China distribution group, and Central and Western China distribution group, respectively. Among Chinese dwarf cherry and its closely related plant species of unambiguous,20 primers screened for amplification amplified 683 and of which 98.99% were polymorphic. UPGMA cluster analysis showed that Chinese dwarf cherry and its closely related plant species accessions were grouped into 3 separate clusters at the genetic similarity value of 0, 61. The largest inheritance distance was between Chinese dwarf cherry and Prunus glandulosa while the least inheritance distance was between Chinese dwarf cherry and peach（Prunus persica）.

关 键 词：欧李 野生种群 RAPD 遗传多样性 近缘植物 聚类分析 

分 类 号：S662.3[农业科学—果树学]