腺嘌呤合成腺苷甲硫氨酸的工程菌构建及其高产株筛选  

Synthesis of S-adenosylmethionine from adenine in constructing engineered strain and the screening of high yield strain

作　　者：郑计瑞 陈丽芬[1] 杨善岩[1] 朱家荣[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《食品与发酵工业》2014年第9期23-28,共6页Food and Fermentation Industries

摘　　要：为了获得腺嘌呤合成腺苷甲硫氨酸(SAM)的高产菌株,将酿酒酵母的SAM合成酶基因sam 2克隆至整合型质粒pCB1004-Pgpd的多克隆位点,获得重组质粒pCB1004-Pgpd-sam 2。在PEG-CaCl2介导下,重组质粒导入至雅致放射毛霉原生质体内,获得SAM工程菌。以腺嘌呤为合成前提物的条件下,通过菌株筛选获得1株高产的SAM工程菌,其SAM产量相对于出发菌株提高了1.5倍。In this paper, Saccharomyces cerevisiae genome was used as a template, and the key enzyme gene sam 2 was cloned into multiple cloning sites of the integrated plasmid pCBlOO4-Pgpd by PCR to obtain the recombinant plasmid for synthesis of SAM. The recombinant plasmid was transformed into Actinomucor elegans protoplast under the mediation of PEG-CaC12 to get SAM productive engineering strain. With the adenine as precursor, the SAM produc- tion of screened strain increased from 0.40 g/L to 1.00 g/L as compared to the original strain, which increased by 1.5 times.

关 键 词：S-腺苷甲硫氨酸 雅致放射毛霉 sam 2 腺嘌呤 

分 类 号：TS201.3[轻工技术与工程—食品科学]