赤藓糖醇生产母液中糖醇组分的定性鉴定  

Identification of the components in the erythritol mother liquor

作　　者：赵祥颖[1] 张立鹤[1] 杨丽萍[1] 韩延雷 田延军[1] 张家祥[1] 刘建军[1] 

机构地区：[1]山东省食品发酵工程重点实验室,山东省食品发酵工业研究设计院,山东济南250013

出　　处：《食品与发酵工业》2014年第10期200-204,共5页Food and Fermentation Industries

基　　金：山东省科技发展项目(No.2011GSF11610)

摘　　要：前期研究表明,赤藓糖醇生产母液中除含有赤藓糖醇外还含有其他未知糖醇组分,该文采用HPLC和LC-MS分析对这些未知糖醇组分进行了定性鉴定。首先使用氨基色谱柱和Shodex SC1011色谱柱相结合对赤藓糖醇母液进行HPLC分析,通过对比两色谱柱对母液和糖醇标准样品的分析结果,初步确定赤藓糖醇母液中的未知糖醇组分主要为阿拉伯糖醇和甘露糖醇,此外还含有少量的核糖醇。LC-MS分析结果显示,母液中各组分分子质量与赤藓糖醇、阿拉伯糖醇(核糖醇)、甘露糖醇一致,进一步确认了HPLC分析结果。The earlier investigation indicated that, in addition to erythritol, the erythritol mother liquor still con- tained other unknown sugar alcohol constituents. In this paper, the high performance liquid chromatography （HPLC） and liquid chromatography-mass spectrometry （LC-MS） analysis were employed in order to qualitatively determine these unknown substances. To be first, the HPLC analysis was undertook using the NH2 column and Shodex SC1011 column, respectively. Based on the comparison between the mother liquor peaks and standard samples peaks, the preliminary conclusions were drawn that the unknown constituents were mainly arabitol and mannitol, as well as a few amounts of ribitol. Furthermore, the LC-MS analysis revealed that their molecular weights were coincided with that of arabitol, mannitol and ribitol. Altogether, the results provided a scientific basis for the further resource utilization of the mother liquor.

关 键 词：赤藓糖醇 母液 HPLC 阿拉伯糖醇 甘露糖醇 

分 类 号：TS202.3[轻工技术与工程—食品科学]