微型丝网印刷电极快速检测克仑特罗的方法  

On-site Electrochemical Detection of Clenbuterol Based on Mini-Screen Printed Electrodes

作　　者：梁桦[1] 宋伟[2] 许丹科[2] 刘英 钟文英[1] 

机构地区：[1]中国药科大学理学院,江苏南京211198 [2]南京大学化学化工学院,江苏南京210093 [3]南京祥中生物科技有限公司,江苏南京210022

出　　处：《食品科学》2014年第8期99-104,共6页Food Science

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB911003);国家自然科学基金面上项目(21175066;21227009);江苏省科技支撑计划项目(BE2011773);江苏省环境监测科研基金项目(1116);国家创新研究群体科学基金项目(21121091)

摘　　要：研制能用于96孔酶标板快速检测的微型丝网印刷电极,并建立克仑特罗的电化学分析方法.通过间接竞争免疫反应与计时电流法相结合实现克仑特罗的残留量的定量测定.实验中考察并优化免疫反应与酶催化反应的条件.结果表明：在优化的条件下,克仑特罗标准溶液质量浓度在0.025～2.0 μg/L范围内呈现良好线性关系（r为0.999 2）.采用本实验方法检测猪肉和猪尿中克仑特罗的结果与商品化ELISA试剂盒一致.本方法检测限分别为0.18 μg/L和0.05 μg/L,比ELISA试剂盒检测限低一个数量级.An electrochemical sensing method for the detection of clenbuterol （CLB） was developed based on miniscreen printed electrodes and 96-well mierotiter plate.Clenbuterol residues were indirectly detected by combined use of indirect competitive immunoreaction and chronoamperometry.The immune reaction and enzymatic reaction conditions were investigated.Under the optimized conditions,the results showed that the electrochemical immunosensor produced a linear range of 0.025-2.0 μg/L with a correlation coefficient of 0.999 2.The analytical results of CLB residues in pork and swine urine samples were consistent with those obtained with an enzyme-linked immunosorbent assay （ELISA） plate.The detection limits for pork and swine urine samples were 0.18 μg/L and 0.05 μg/L,respectively,showing a decrease of one order of magnitude in comparison with those of ELISA.

关 键 词：微型丝网印刷电极 克仑特罗 计时电流法 电化学免疫传感 

分 类 号：TS207.3[轻工技术与工程—食品科学]