基于纳米金电化学免疫传感器测定牛奶中的青霉素G  

Determination of Penicillin G in Milk by Gold Nanoparticles Based Electrochemical Immunosensor

作　　者：李建龙[1] 潘道东[1] 朱浩嘉[1] 顾愿愿 赵紫微[1] 朱珊珊[1] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《食品科学》2014年第8期111-114,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2012BAK08B01);宁波市创新团队项目(2012B82017);宁波市人事局人才基金项目(ZX2012000380)

摘　　要：利用吸附法将青霉素G抗体固定于纳米金修饰的玻碳电极表面,制备用于检测青霉素G的电化学免疫传感器,建立高度灵敏的一步直接电化学免疫法.纳米金的强吸附和导电作用,提高了青霉素G抗体的固定量和电化学灵敏度.在优化条件下,该传感器的响应电流与青霉素质量浓度的对数在0.04～40.00 ng/mL范围内呈良好的线性关系,相关系数为0.988 4,检测限为2.49 ng/mL,该法成功的实现了对牛奶中青霉素G的检测.An amperometric immunosensor was fabricated by co-immobilizing penicillin G polyclonal antibody （Ab＊） on the surface of a gold nanoparticle modified glassy carbon electrode through electrostatic adsorption.A highly sensitive one-step direct electrochemical immunoassay was established due to the strong adsorption and conductive capacity of gold nanoparticles,thus raising the amount of fixed penicillin G antibody and electrochemical sensitivity.Under optimized conditions,the current response of the sensor showed a good linear relationship with logarithmic penicillin concentration in the range of 0.04-40.00 ng/mL with a correlation coefficient of 0.988 4 and the detection limit was 2.49 ng/mL.The method was able to detect penicillin in milk successfully.

关 键 词：纳米金 青霉素 电化学免疫传感器 

分 类 号：TS201.2[轻工技术与工程—食品科学]