鮟鱇鱼皮胶原蛋白肽的抗氧化活性  

Antioxidant Activity of Collagen Peptides from Lophius litulon Skin

作　　者：马华威[1] 杨会成[2,3] 付万冬 廖妙飞 周宇芳 相兴伟 陈孟[1] 郑斌 

机构地区：[1]浙江海洋学院海洋科学学院,浙江舟山316022 [2]浙江省海洋开发研究院,浙江舟山316021 [3]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品科学》2014年第9期80-84,共5页Food Science

基　　金：浙江省厅市会商项目(2011C02003);舟山市海洋类项目(2011C21059);浙江省公益技术研究农业项目(2012C22068);"十二五"国家科技支撑计划项目(2012BAD28B05-02)

摘　　要：选取铁离子还原体系和3种自由基体系为指标,研究鮟鱇鱼皮胶原蛋白肽体外清除自由基效果和抗氧化作用。采用超滤膜分离鮟鱇鱼皮胶原蛋白肽组分,筛选出体外清除·OH活性最好的组分,再建立小鼠衰老模型,将分离得到活性最好的组分采用灌胃法,测定小鼠皮肤中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)及丙二醛(malondialdehyde,MDA)和羟脯氨酸(hydroxyproline,Hyp)的含量,研究鮟鱇鱼皮胶原蛋白肽体内抗氧化和清除自由基作用。结果表明:鮟鱇鱼皮胶原蛋白肽具有较强的还原能力且对O-2·、DPPH自由基、·OH具有清除效果,质量浓度为10 mg/mL时对·OH、DPPH自由基、O-2·的清除率和对Fe3+的还原能力分别为70.48%、68.78%、42.53%和0.676;分子质量小于2 000 D的鮟鱇皮胶原蛋白肽组分对·OH具有较好的清除效果,IC50为15.7 mg/mL;剂量为100 mg/(kg?d)时,显著提高了皮肤中SOD、GSH-Px、CAT的活性,较模型组分别增加20.9%、41.3%和58.1%,且显著抑制MDA的形成。A ferric ion-reducing system and three free radical systems were used to evaluate the radical-scavenging capacity and antioxidant activity of collagen peptides from Lophius litulon skin in vitro. The peptide components were separated by ultrafiltration and the fraction with the highest hydroxyl radical scavenging activity in vitro was obtained. In addition, aged mouse models were constructed and determined for SOD, CAT, GSH-Px, MDA and Hyp contents in the skin after oral administration of the screened fraction. The results showed that Lophius litulon skin collagen peptides had strong reducing power and superoxide anion, DPPH and hydroxyl radical scavenging capacity in vitro. When the sample concentration was 10 mg/mL, the scavenging rates of hydroxyl, DPPH and superoxide anion radicals and the ferric ion reducing power were 70.48%, 68.78%, 42.53% and 0.676, respectively. The fraction with relative molecular weight less than 2 000 D had stronger hydroxyl radical scavenging activity, and its ICs0 was 15.7 mg/mL. The activities of SOD, GSH-Px and CAT in skin were increased by respectively 20.9%, 41.3% and 58.1% at the dose of 100 mg/（kg＇d） when compared with the model group. Moreover the generation of MDA was significantly inhibited.

关 键 词：鮟鱇鱼皮 胶原蛋白肽 抗氧化 丙二醛 羟脯氨酸 

分 类 号：Q936.16[生物学—微生物学]