芦蒿秸秆黄酮类化合物对晚期蛋白质糖基化终末产物形成的抑制作用  

Inhibitory Effect of Artemisia selengensis Straw Flavonoids on the Formation of Advanced Glycation End Products(AGEs)

作　　者：邓荣华[1,2] 陆敏[1] 夏秋琴 李晓明[1] 孔阳辉 吕丽爽[1] 

机构地区：[1]南京师范大学金陵女子学院,江苏南京210097 [2]青岛商务学校,山东青岛266002

出　　处：《食品科学》2014年第9期123-127,共5页Food Science

基　　金：江苏省普通高校研究生科研创新计划立项项目(CXLX11_0893;CXLX12_0417);江苏省自然科学基金项目(BK2012850);江苏省高校自然科学基金项目(12KJB5500005);浙江省自然科学基金项目(LY12C15001)

摘　　要：通过建立牛血清白蛋白-丙酮醛(bovine serum albumin-methylglyoxal,BSA-MGO)的蛋白质糖基化反应模型,用荧光法测定体外晚期蛋白质糖基化终末产物(advanced glycation end products,AGEs)的含量,探讨芦蒿秸秆黄酮类化合物对AGEs形成的抑制效果,并对木犀草素的作用途径进行探索。结果表明:芦蒿秸秆总黄酮浸膏经AB-8树脂分离后共收集到4组含有黄酮的洗脱组分(F-10、F-30、F-50、F-70)对AGEs的形成均具有抑制作用,抑制效果由强到弱为依次为F-50>F-30>F-10>F-70,此结果与F-30和F-50分离纯化得到的黄酮成分芦丁和木犀草素具有较好的AGEs抑制效果相一致。同时发现,芦蒿秸秆对AGEs形成的抑制效果与总黄酮含量存在显著的线性关系。另外,将木犀草素与MGO的反应产物进行分离纯化,高效液相色谱-质谱联用分析显示:木犀草素作用途径为通过捕获MGO,形成木犀草素-MGO加和物来抑制AGEs的形成。芦蒿秸秆黄酮类化合物可抑制体外蛋白质糖基化反应的活性预示其可以作为AGEs的天然抑制剂来预防和减轻糖尿病及其并发症。The inhibitory activity of flavonoids from Artemisia selengensis straw against the formation of fluorescent advanced glycation end products （AGEs） was evaluated in a bovine serum albumin-methylglyoxal （BSA-MGO） model. The formation of total AGEs could be generally followed by measuring their characteristic fluorescence at the excitation and emission maxima of 370 and 440 nm, respectively. Results indicated that 4 elution fractions （F-10, F-30, F-50 and F-70） obtained with AB-8 resin from the flavonoid extract of Artemisia selengensis straw revealed significant inhibitory activities against the formation of AGEs in decreasing order： F-50 〉 F-30 〉 F-10 〉 F-70, which was consistent with the inhibitory effects of rutin and luteolin purified from F-30 and F-50 against the formation of AGEs. Meanwhile, the inhibitory activities of elution fractions were found to be linearly correlated with their total flavonoid contents. The adduct formed from the reaction between luteolin and MGO was purified, and LC-MS analysis showed that luteolin might have the potential to inhibit the formation of AGEs by trapping MGO to form a di-MGO conjugated adduct. Our findings showed that the flavonoids from Artemisia selengensis straw could inhibit the formation of AGEs in vitro, implying their potential use as a new inhibitor for preventing and alleviating diabetes and diabetes-related complications.

关 键 词：芦蒿秸秆 黄酮类化合物 蛋白质糖基化终末产物 

分 类 号：TS201.2[轻工技术与工程—食品科学]