蚕豆花左旋多巴提取工艺优化  

Optimization for the Extraction Process of Levodopa from Broad Bean Flowers

作　　者：徐晓俞[1] 李爱萍[1] 吴凌云 郑开斌[1,3] 

机构地区：[1]福建省农业科学院作物研究所,福州350013 [2]福建省农田建设与土壤肥料技术总站,福州350003 [3]福建省农业科学院甘蔗研究所,福建漳州363005

出　　处：《中国农学通报》2014年第34期56-60,共5页Chinese Agricultural Science Bulletin

基　　金：福建省农业科学院青年科技创新基金"蚕豆花左旋多巴提取工艺优化与应用"(2012DQA-5);福建省财政专项--福建省农业科学院科技创新团队建设项目"粮经作物种质创新及其利用"(CXTD-1-1301);福建省农业科技计划重点项目"蚕豆花左旋多巴提取技术研究及其应用"(2014N0024)

摘　　要：为了提高蚕豆花左旋多巴的提取得率,采用加热回流提取法优化蚕豆花左旋多巴提取工艺。通过单因素和正交试验设计,考察液料比、溶剂p H、提取时间对蚕豆花左旋多巴提取得率的影响,确定最佳提取工艺条件为:液料比20:1,溶剂p H 4.5,提取时间2 h,在此条件下左旋多巴提取得率为3.67%,相比冷浸法提高了3.67%。本工艺中使用乙醇来调节溶剂的p H值,可以抑制水溶性杂质的析出,经HPLC谱图分析,提取物中杂质组分较少,且左旋多巴与杂质组分分离较好,这有利于左旋多巴进一步的纯化。本研究可为从蚕豆花中工业化提取左旋多巴提供技术依据。In order to improve the extraction rate of levodopa from broad bean flowers, the extraction process was optimized by heating reflux extraction. Through single factor and orthogonal test design, the effects of the ratio of liquid to material, solvent pH and extraction time on the extraction yield of levodopa from broad bean flowers were investigated. The optimum extraction conditions were determined as follow： the ratio of liquid to material 20：1, solvent pH 4.5, extraction time 2 h. Under the optimized conditions, the extraction rate of levodopa was 3.67%, which was 3.67% higher than cold maceration. The ethanol was used to adjust the pH value of solvent in the process, which could inhibit the precipitation of the water soluble impurities. The impurity components were less in the extract and the separation of levodopa and impurities was good by HPLC, so it＇ s conducive to the further purification of levodopa. The study can provide a technical basis for the industrial extraction of levodopa from broad bean flowers.

关 键 词：蚕豆花 左旋多巴 加热提取 正交试验 

分 类 号：TS201[轻工技术与工程—食品科学]