肝细胞癌基因组5q13.2与8p23.1位点的杂合性缺失分析  

Loss of Heterozygosity in Two Distinct Regions,5q13.2 and 8p23.1 in Hepatocellular Carcinoma

作　　者：赵璋[1] 张宁[1] 龙江[2] 张蓓[1] 何肖敏[1] 黄坚[1] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050 [2]首都医科大学附属北京佑安医院,北京100069

出　　处：《肿瘤学杂志》2015年第1期34-38,共5页Journal of Chinese Oncology

基　　金：王宝恩肝纤维化基金(20100013);北京市自然科学基金(7132058)

摘　　要：[目的]分析肝细胞癌(HCC)中5q13.2和8p23.1位点的杂合性缺失(LOH)情况,以探索新的抑癌基因。[方法]应用聚合酶链反应—非变性聚丙烯酰胺凝胶电泳法对83例HCC患者基因组DNA中的5q13.2和8p23.1位点的LOH进行检测,并将其与临床参数进行关联分析。[结果]83例HCC患者基因组中,36.1%(30例)存在5q13.2位点LOH,在该位点存在OCLN、SMN2、SERF1A、SMN1、NAIP和GTF2H2基因;8p23.1的D8S503和D8S1130位点分别存在68.4%和61.3%的LOH。[结论]HCC患者基因组中存在较高频率的5q13.2和8p23.1位点LOH,这些位点中可能存在与HCC发生密切相关的基因。[Purposel To investigate the frequency of loss of heterozygosity （LOH） at 5q13.2 and 8p23.1 in hepatocellular carcinoma （HCC）,and to explore possible tumor suppressor genes in the loci.[Methods] The frequency of LOH at 5q13.2 and 8p23.1 were analyzed in 83 cases with HCC by polymerase chain reaction-polyacrylamide gel electrophoresis. Correlation analysis were performed between the frequency of LOH and clinicopathological characteristics of HCC. [Results] Thirty of 83 HCC cases were indentified with LOH（36.1%） at 5ql3.2,where a series of functional genes were harbored such as OCLN, SMN2, SERF1A, SMN1, NAIP and GTF2H2. The frequencies of LOH at D8Sl130 and D8S503 in 8p23.1 were 61.29% and 68.4% respectively.[Conclusion] In the genome of HCC,there are high frequency of LOH at 5q13.2 and 8p23.1 ,where critical genes associated with the development of HCC may harbor.

关 键 词：杂合性缺失 肝细胞癌 基因 

分 类 号：R735.7[医药卫生—肿瘤]