HPLC法同时测定不同产地大花红景天中6种活性成分  

HPLC method for simultaneous quantitative determination of six active ingredients in Rhodiola crenulata from different origins of Qinghai-Tibet Plateau

作　　者：刘青[1,2] 杜守颖[1] 多吉仁青 尼玛次仁[2] 顿珠[2] 嘎务[2] 

机构地区：[1]北京中医药大学,北京100102 [2]西藏藏医学院,西藏拉萨850000

出　　处：《中草药》2015年第2期276-279,共4页Chinese Traditional and Herbal Drugs

基　　金："973"计划前期研究专项(2012CB722905)

摘　　要：目的对青藏高原不同产地大花红景天Rhodiola crenulata中没食子酸、红景天苷、酪醇、儿茶素、没食子酸乙酯、对香豆酸进行分析研究,建立定量分析方法,为其进一步利用提供依据。方法采用30%乙醇提取样品;Diamonsil C18色谱柱（250 mm×4.6 mm,5μm）;以乙腈-0.3%磷酸溶液为流动相进行梯度洗脱;检测波长275 nm,柱温25℃。结果没食子酸、红景天苷、酪醇、儿茶素、没食子酸乙酯、对香豆酸分别在38.2-382.0 ng/m L（r=0.999 8）、301.0-3 010.0μg/m L（r=0.999 9）、19.8-198.0 ng/m L（r=0.999 6）、17.1-171.0 ng/m L（r=0.999 6）、4.5-45.0 ng/m L（r=0.999 8）、6.38-63.80 ng/m L（r=0.999 4）呈良好的线性关系,加样回收率均符合定量测定要求。结论该方法简便,快速,准确,可用于同时对青藏高原大花红景天中6种活性成分的测定。Objective To analyze the six active ingredients in Rhodiola crenulata from the different origins of Qinghai-Tibet Plateau,to establish quantitative analysis methods,and to provide a basis for further use.Methods Samples were extracted with 30% ethanol;In Diamonsil C18 column（250 mm × 4.6 mm,5 μm）,gradient elution was carried out with acetonitrile-0.3% phosphoric acid solution as the mobile phase,detection wavelength 275 nm,and column temperature 25 ℃.Results A good linear relationships of gallic acid,salidroside,tyrosol,catechin,ethyl gallate,and coumaric acid were at 38.2—382.0（r = 0.999 8）,301.0—3 010.0（r = 0.999 9）,19.8 —198.0（r = 0.999 6）,17.1—171.0（r = 0.999 6）,4.5—45.0（r = 0.999 8）,and 6.38—63.80 ng/m L（r = 0.999 4）,respectively.Conclusion The method is simple,rapid,accurate,and can be used simultaneously to determine the content of the six active ingredients in R.crenulata from the different origins of Qinghai-Tibet Plateau.

关 键 词：大花红景天 没食子酸 红景天苷 酪醇 儿茶素 对香豆酸 没食子酸乙酯 

分 类 号：R286.022[医药卫生—中药学]