响应面法优化香菇多糖的超声辅助提取工艺  

Optimization of Ultrasonic Assisted Extraction of Lentinan by Response Surface Methodology

作　　者：万阅 齐计英 曾红[1] 韩阳[2] 韩静[1] 

机构地区：[1]沈阳药科大学制药工程学院,沈阳110016 [2]辽宁中医药大学药学院,沈阳110032

出　　处：《生物技术通报》2015年第1期79-85,共7页Biotechnology Bulletin

摘　　要：为了开发利用香菇资源,采用超声辅助法提取香菇中的多糖,利用响应面法优化超声辅助提取法提取香菇多糖的工艺条件。首先进行单因素试验考察,在单因素试验的基础上,选择超声波功率、超声时间及料液比为自变量,以多糖得率为响应值,采用Box-Benhnken法设计3因素3水平响应面设计试验。结果表明,响应面模型与实际情况拟合良好,能较好地预测香菇多糖得率。最佳工艺:超声波功率300 W、超声波处理时间25 min、料液比1∶30,多糖得率25.71%,与理论值(25.55%)相比,相对误差较小,为0.63%。与传统热水浸提法比较,超声波提取法多糖得率较高,且耗时少,是理想的香菇多糖提取方法。Technological conditions of extraction ofLentinan with ultrasonic extraction are optimized for developing and utilizing the resource of Mushrooms by using the response surface method（RSM）. Firstly, single factor experiment was investigated, and then based on single factor experiment, a 3-factor, 3-level Box-Benhnken experiment was designed, in which ultrasonic power, ultrasonic time and the ratio of solid to liquid were chosen as influencing factors, and the yield of polysaccharide was selected as response value. The result shown that the regression model fit well with experimental data, and it can well predict the polysaccharide yield of Lentinan. The optimum conditions of ultrasonic extraction were ultrasonic power of 300 W, ultrasonic extraction time of 25 min and solid-to liquid ratio of 1∶30, and the polysaccharide yield was 25.71%. Compared with the theoretical value（25.55%）, it has a smaller relative error at 0.63%. Compared with traditional method, ultrasonic extraction method is more efficient and less time consuming, so it is the ideal extraction method of Lentinan.

关 键 词：热水浸提 超声辅助提取 多糖得率 响应面优化 单因素试验 

分 类 号：TQ281[化学工程—有机化工]