脑创伤后Edaravone脑保护作用机制的实验研究  

Experimental study of the protective effect of Edaravone on brain after traumatic brain injury

作　　者：尹立国[1] 崔建忠 高俊玲[3] 赵雅宁[3] 

机构地区：[1]河北省遵化市人民医院,064200 [2]河北省唐山市工人医院 [3]河北联合大学

出　　处：《河北医药》2015年第3期353-355,共3页Hebei Medical Journal

摘　　要：目的探讨Edaravone对脑创伤后保护作用的可能机制。方法采用改进的Marmarou’s法建立大鼠中度闭合性弥漫性颅脑损伤模型,应用TAB法、免疫组化,TUNEL法对大鼠伤后皮质自由基、细胞色素C及凋亡细胞数进行动态观察。结果伤后给予Edaravone能明显减少自由基水平,降低细胞色素c表达和神经元细胞凋亡数。结论 Edaravone对颅脑创伤有治疗作用,其机制之一是通过减少自由基生成,抑制线粒体膜通透性转换孔(MPTP)开放,减少细胞色素c释放,进而减少神经细凋亡。Objective To investigate the possible action mechanism of protective effect of Edaravone on brain after traumatic brain injury .Methods The middle degree closed diffused craniocerebral injury models in rats were established by improved Marmarou＇s method.After the models were successfully established ,the free radical in cortex of the rats ,Cyt-c and apoptosis cell numbers were detected and observed dynamically by TAB ,immunohistochemry,TUNEL,respectively.Results Edaravone could decrease obviously the levels of free radical ,the expression of Cyt-c and apoptosis nerve cells after traumatic brain injury Conclusion Edaravone is effective in treating traumatic brain injury ,and one of action mechanisms is to reduce generation of free radical,inhibit opening of mitochondrial permeability transition pore （MPTP）,reduce releasing of Cytc,and then decrease nerve cell apoptosis .

关 键 词：脑创伤 EDARAVONE 自由基 线粒体膜通透性转换孔 细胞色素C 凋亡 

分 类 号：R749.12[医药卫生—神经病学与精神病学]