基于分子互作网络的MMPs相关基因在胃癌组织中的表达研究  

Expression analysis of MMPs and their related genes based on the molecular interaction network in gastric cancer tissues

作　　者：许建婷 金浩范[2] 王冀邯[1] 牛超[2] 高长曌 王国庆[1] 李凡[1] 

机构地区：[1]吉林大学基础医学院,吉林长春130021 [2]吉林大学第一医院肿瘤中心,吉林长春130021

出　　处：《中国实验诊断学》2015年第1期56-59,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨MMPs相关分子互作网络在胃癌组织中的表达情况。方法运用10对胃癌及相应癌旁组织芯片筛选胃癌组织中差异表达基因,结合BioGRID数据库,构建胃癌组织中表达异常的MMPs相关分子互作网络。结果胃癌中多个MMPs家族成员及其互作网络中的18个基因均呈高表达状态,主要参与的生物学过程及通路和癌症细胞的侵袭、转移等过程密切相关。结论高通量组学及相关数据库、分析工具的结合有助于构建癌症中表达异常的分子互作网络,为后续的研究提供较全面的分子学机制基础。Objective To explore the expression of MMPs-related molecular interaction network in gastric cancer tissues.Methods 10 pairs of gastric cancer and their corresponding para-carcinoma tissues were analyzed with microar-ray for screening of the differentially expressed genes,then constructed the differentially expressed MMPs-related mo-lecular interaction network in gastric cancer tissues by integrating the information from BioGRID databases and the mi-croarray results.Results 1 8 genes in the MMPs-related molecular interaction network （also including several MMPs members）were up-regulated in gastric cancer tissues,the main biological processes and pathways that they participated in were closely related with cancer invasion and metastasis progression.Conclusion The combination of high-through-put genomics,bioinformatic databases and analytical tools is necessary for the analysis of cancer-related molecular inter-action network,which will provide more comprehensive molecular mechanisms for the future research.

关 键 词：分子互作网络 MMPS BioGRID数据库 GO PATHWAY 

分 类 号：R735.2[医药卫生—肿瘤]