香荚兰豆的TLC鉴别及香兰素、对羟基苯甲醛的含量测定  

Identification of Vanillae Fructus by TLC and Determination of Vanillin and P-Hydroxybenzaldehyde

作　　者：李肖爽[1] 卢金清[1] 戴艺[1] 梁欢[1] 李雨玲[1] 黎强[1] 

机构地区：[1]湖北中医药大学湖北省药用植物研发中心,湖北武汉430065

出　　处：《食品研究与开发》2014年第23期97-101,共5页Food Research and Development

摘　　要：采用薄层色谱法(TLC)对香荚兰豆原料进行了定性鉴别,并用高效液相色谱法(HPLC)同时测定香荚兰豆药材中香兰素和对羟基苯甲醛的含量。TLC法:采用正丁醇-无水乙醇-浓氨水(7∶2∶1)为展开剂,置紫外灯(254 nm)下检视。HPLC法:采用Agilent ZORBAX SB-C18(250 mm×4.6 mm,5μm)色谱柱;以甲醇-水为流动相梯度洗脱;体积流量1.0 mg/m L;检测波长分别为275 nm;柱温25℃。TLC中斑点清晰可见。香兰素线性范围为0.408μg^0.816μg(r=0.999 8),平均回收率为100.13%,RSD为1.75%;对羟基苯甲醛线性范围为0.004μg^0.020μg(r=0.9999),平均回收率为99.56%,RSD为1.20%。所测香荚兰豆中,香兰素和对羟基苯甲醛的平均含量分别为7.26%、0.37%。以上方法可靠、准确、重复性好,适用于马达加斯加香荚兰豆的鉴别及其香兰素及对羟基苯甲酸及含量测定。Thin-layer chromatography was introduced as quality identification for Vanillae Fructus raw material and an HPLC method was established for the content determination of Vanillin and P-Hydroxybenzaldehyde. TLC：The mobile phase was butyl alcohol-absolute alcohol-ammonia liquor （7∶2∶1）,the ultraviolet detection was carried out at 254 nm. HPLC： The separation was performed on Agilent ZORBAX SB-C18 （250 mm× 4.6 mm,5 μm） with the mobile phases of methanol-water. The flow rate was 1.0 mg/mL, the detection wavelengths were set at 275 nm and the column temperature was maintained at 25℃. Spots in TLC were visible. The linear ranges of Vanillin was in a range of 0.408μg-0.816μg （r=0.999 8）, the average recoveries were 100.13%with a RSD of 1.75%. The linear ranges of Hydroxyben-zaldehyde was in a range of 0.004μg-0.020μg （r=0.999 9）, the average recoveries were 99.56%with a RSD of 1.20%.The average contents of Vanillin and p-Hydroxyben- zaldehyde were 7.26 % and 0.37 % respectively. The method is simple, reliable with a good reproducibility and is applicable for the determination of Vanillin and P-Hydroxybenzaldehyde in Vanillae Fructus raw material.

关 键 词：香荚兰豆 薄层色谱法 高效液相色谱法 香兰素 对羟基苯甲醛 

分 类 号：O657.72[理学—分析化学] TS214[理学—化学]