液相色谱串联质谱同时检测鸡肉中金刚烷胺、氟喹诺酮类和磺胺类药物的残留  

Simultaneous determination of amantadine,fluoroquinolones and sulfonamides residues in chicken by LC-MS/MS

作　　者：饶钦雄[1] 郭黎明[2] 周苏[1] 罗爱琼[1] 刘丹[1] 赵志辉[1] 

机构地区：[1]上海市农业科学院农产品质量标准与检测技术研究所,上海201403 [2]北京华都肉鸡公司检测中心,北京102211

出　　处：《上海农业学报》2014年第6期102-106,共5页Acta Agriculturae Shanghai

基　　金：2014年度上海市农业科学院青年人才成长计划[沪农青字(2014)第1-6号]

摘　　要：采用液相色谱串联质谱法(LC-MS/MS)测定了鸡肉中金刚烷胺、4种氟喹诺酮类和6种磺胺类药物的残留。试样用甲醇水溶液(7/3,V/V)提取,经混合型阳离子固相萃取柱净化,由LC-MS/MS进行定性定量分析。该法对所有检测药物的检出限均为1μg/kg,定量限为5μg/kg o添加水平为5μg/kg、10μg/kg和50μg/kg时,1 1种药物的添加回收率为60.6%—109.0%,变异系数为2.1%—13.9%。A method was established to determine the residues of amantadine,4 kinds of fluoroquinolones and 6 kinds of sulfonamides in chicken by LC-MS/MS.The sample was extracted with methanol/water（7/3,V/V）solution,and then the solution was purified through a MCX SPE cartridge and qualitatively and quantitatively analyzed by LC-MS/MS.The detection limits of all drugs were1 μg/kg and their quantification limits were 5 μg/kg.When the addition levels were 5 μg/kg,10 jug/kg and 50 μg/kg,the recovery rates of the above 11 drugs were 60.6%—109.0%,and the variation coefficients were 2.1%—13.9%.

关 键 词：鸡肉 残留 金刚烷胺 氟喹诺酮 磺胺 液相色谱串联质谱 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]