响应面法优化金银花多糖羧甲基化工艺及抗氧化性研究  

Preparation of Carboxymethyl Lonicera japonica Polysaccharides and Its Antioxidant Ability

作　　者：赵鹏[1] 张婷婷[1] 

机构地区：[1]陕西中医学院药学院,咸阳712046

出　　处：《天然产物研究与开发》2015年第1期114-119,190,共7页Natural Product Research and Development

基　　金：陕西省自然科学基金项目(2011JQ2005);陕西省教育厅专项项目(11JK0691)

摘　　要：用响应面法研究了金银花多糖羧甲基化的最优合成工艺,以多糖羧甲基化的取代度为评价指标,考察了反应时间、氯乙酸浓度和反应温度对多糖羧甲基化的取代度的影响。通过响应面法得到的最优工艺条件为:反应时间是2.6 h,氯乙酸浓度是3.2 mol/L,反应温度是60℃。初步的抗氧化性实验表明,羧甲基化后的金银花多糖的抗氧化性能有了明显提高。In this study,the synthetic conditions of Lonicera japonica polysaccharides carboxymethylation derivatives were investigated. The influences of reaction time,concentration of chloroacetic acid and reaction temperature on the degree of substitution（DS） of the synthesized derivatives were optimized by response surface methodology（RSM）. The optimal conditions were reaction time of 2. 6 h,concentration of chloroacetic acid of 3. 2 mol/L and reaction temperature of 60℃. The degree of substitution was 0. 793. Compared with L. japonica polysaccharides,the antioxidant ability of the synthesized carboxymethylation derivatives was improved.

关 键 词：金银花多糖 羧甲基化 响应面 抗氧化性 

分 类 号：R283.6[医药卫生—中药学]