两种纤维素酶水解漂白针叶木纤维能力的比较  

Comparison of the hydrolysis capacity of two kinds of cellulase

作　　者：杜敏[1] 李新平[1] 于长青 陈立红[3] 王志杰[1] 李鸿魁[1] 

机构地区：[1]陕西科技大学轻工与能源学院陕西省造纸技术及特种纸品开发重点实验室,陕西西安710021 [2]广州赛锐化工有限公司,广东广州510640 [3]陕西科技大学化学与化工学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2015年第1期21-24,29,共5页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金项目(31370578);陕西省科技厅重点实验室科研项目(2011HBSZS014);陕西省科技厅自然科学基础研究计划项目(2014JZ013);陕西科技大学博士科研启动基金项目(BJ13-21)

摘　　要：采用复合纤维素酶Celluclast 1.5L和内切纤维素酶Novozym 476等分别对漂白针叶木纤维进行了水解实验.通过对比两种酶的酶活力大小以及水解后纤维得率的变化情况,分析比较了两种纤维素酶水解纤维素纤维的能力大小.其结果显示:纤维素酶Celluclast 1.5L具有较高的滤纸酶活,而纤维素酶Novozym 476具有较高的CMC酶活.经纤维素酶Celluclast 1.5L水解处理后,随着酶用量的增加或酶处理时间的延长,纤维得率急速下降,当酶用量为20.0FPU/g、水解时间为2h时,纤维得率约为81%;当酶用量为10.0FPU/g、水解时间为48h时,纤维得率仅为55.34%,这说明纤维素酶Celluclast 1.5L对纤维素具有很强的水解作用,它不仅可以作用于纤维素的无定形区,同时也可以作用于纤维素的结晶区.纤维素酶Novozym 476亦能使纤维素发生一定程度的水解,但催化水解能力远低于纤维素酶Celluclast 1.5L,当Novozym 476用量为50.0CMCU/g时,仅有5%左右的纤维素水解成为葡萄糖.Composite cellulase named as Celluclast 1.5Land endo cellulase named as Novozym 476 were used to hydrolyze bleached softwood fiber respectively.By comparing the enzyme activity and the change of the fiber yield after enzymatic hydrolysis,the ability of the hydrolysis of the two cellulases was analyzed and compared.The results showed that Celluclast 1.5Lhas a higher filter activity,and Novozym 476 has a higher CMC activity.Hydrolyzed by Celluclast 1.5L,with the increasing of the enzyme dosage or enzyme treatment time,the fiber yield decreased rapidly.When the enzyme dosage was 20.0FPU/g,hydrolysis time was 2h,the fiber yield was about 81%;when the enzyme dosage was 10.0FPU/g,hydrolysis time was 48 h,the fibers yield was only 55.34%,and it indicated that Celluclast 1.5L had stronger hydrolysis ability to cellulose.It could be applied to not only the amorphous regions of cellulose,and also acts on the cellulose crystalline regions.Novozym 476 could also make the cellulose hydrolyze to a certain extent,but the ability of catalytic hydrolysis was far below of Celluclast 1.5L.When Novozym 476 in an amount of 50.0CMCU/g,where only about 5% of cellulose hydrolyzed to glucose.

关 键 词：纤维素酶 漂白针叶木纤维 酶活力 水解能力 

分 类 号：TQ353.1[化学工程]