河豚毒素的样品前处理与快速检测技术研究进展  

Current Progress in Sample Pretreatment and Rapid Detection Methods for Tetrodotoxin

作　　者：方国锋[1] 王锡昌[1] 陶宁萍[1] 辛少平[1] 卢瑛[1] 

机构地区：[1]上海海洋大学食品学院农业部水产品贮藏保鲜质量安全风险评估实验室(上海)上海水产品加工及贮藏工程技术研究中心,上海201306

出　　处：《分析测试学报》2014年第12期1447-1452,共6页Journal of Instrumental Analysis

基　　金：上海市科委部分地方院校计划项目(08390513900);上海市科技兴农重点攻关项目(沪农科攻字(2010)第2-6号);上海市科委工程中心建设项目(11DZ2280300);上海高校一流学科建设项目

摘　　要：河豚毒素是一种毒性极强的小分子、非蛋白神经毒剂,其性质相对稳定,缺乏特效解毒剂。其检测技术主要有生物检测法、理化检测法和免疫学检测法,其中免疫检测因快速、简便、灵敏而得到较为广泛的应用。该文综述了河豚毒素的样品前处理技术、免疫学快速检测技术及相关专利的研究进展情况,并对河豚毒素检测产品的开发进行了展望。Tetrodotoxin（TTX）is a highly toxic low-molecular and non-protein neurotoxin,and its property is relatively stable.There is no specific antidote for it.The three main detection technologies for tetrodotoxin are bioassays,physicochemical detection methods and immunoassays,among which the immunoassay is more widely used because of its celerity,conciseness and delicacy.In this paper,advances in technology of sample pretreatment for tetrodotoxin,rapid immunoassay technology for tetrodotoxin and its related patents were reviewed.The new techniques for tetrodotoxin detection was prospected.

关 键 词：河豚毒素 快速检测 样品前处理 免疫检测 单克隆抗体 

分 类 号：O657.1[理学—分析化学] S852.44[理学—化学]