人脐带间充质干细胞致瘤性试验  

Tumorigenicity Test of Human Umbilical Cord Mesenchymal Stem Cells

作　　者：许键炜[1,2] 何志旭[1,3] 舒莉萍[1,4] 王凤昌[1] 

机构地区：[1]贵阳医学院组织工程与干细胞实验中心,贵州贵阳550004 [2]贵阳医学院药理学教研室,贵州贵阳550004 [3]贵阳医学院附院儿科,贵州贵阳550004 [4]贵阳医学院免疫学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第1期17-19,23,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(No.30560159);贵州省"125计划"重大科技专项[黔教合重大专项字(2013020)];贵州省教育厅自然科学研究项目[黔教合KY字(2012036)]

摘　　要：目的:观察人脐带间充质干细胞对裸鼠的致瘤性。方法:培养扩增人脐带来源的间充质干细胞,制备成细胞生理盐水混悬液注射裸鼠。观察8周裸鼠体内有无肿瘤生长。结果:裸鼠注射细胞后无明显不适,8周观察期内裸鼠体内未见肿瘤生长,病理组织学检测未见异常。结论:人脐带间充质干细胞导致肿瘤发生的可能性低。Objective： To investigate nude mice＇s tumorigenicity caused by human umbilical cord mesenehymal stem cells （HUCMSCs）. Methods： Cultured and amplified HUCMSCs, and prepared in- to cell normal saline hypodermic injection used for injecting nude mice. Tumor growth condition was observed for eight weeks. Results： Mice had no obvious adverse reaction, 8 weeks of tumorigenicity study showed that no tumor developed. Histopathologic examinations also showed no abnormality. Con- clusions： The possibility of HUCMSCs lead to tumor growth is very low.

关 键 词：脐带 间充质干细胞 细胞 培养的 小鼠 裸 免疫组织化学 致癌性试验 

分 类 号：R733.5[医药卫生—肿瘤]