Sema3A在胃癌增殖和侵袭中作用的初步研究  

Preliminary study of the effect of Sema3A on proliferation and invasion of gastric cancer

作　　者：骞佩 王亚芳[1] 庞海林[1] 张贺龙[1] 刘理礼[1] 

机构地区：[1]第四军医大学附属唐都医院肿瘤科,陕西西安710038

出　　处：《现代肿瘤医学》2015年第3期318-322,共5页Journal of Modern Oncology

摘　　要：目的:探讨Sema3A在胃癌增殖和侵袭中的作用。方法:构建Sema3A正义表达载体,经慢病毒包装,感染胃癌高转移潜能MKN-28M细胞,获得外源性稳定表达的Sema3A胃癌细胞株。利用MTT法、克隆形成实验、细胞凋亡实验观察Sema3A过表达对胃癌细胞生长增殖能力的影响。Transwell侵袭实验观察Sema3A过表达对胃癌细胞体外侵袭转移能力的影响。结果:慢病毒包装Sema3A正义表达载体转染入胃癌细胞MKN-28M后,有效增加了Sema3A基因的表达。Sema3A慢病毒包装载体感染MKN-28M细胞后,与对照组相比,细胞生长、增殖速度明显减慢(P<0.05),克隆形成率明显下降(P<0.05),细胞凋亡明显增加(P<0.05),体外侵袭试验表明外源性上调Sema3A显著降低了高转移细胞系MKN-28M的侵袭转移能力(P<0.05)。结论:成功构建了外源性过表达Sema3A基因的慢病毒载体,上调Sema3A基因的表达显著降低了胃癌高转移系MKN-28M细胞的生长增殖能力,抑制了侵袭转移能力,提示Sema3A在抑制胃癌的发生发展中具有重要作用。Objective：To investigate the effects of Sema3A on proliferation and invasion of gastric cancer. Meth- ods：To construct Sema3A sense expression vector,we infected the high metastasis potential MKN -28M cells of gas- tric cancer through the lenti -virus packaging,then obtained exogenous stable expression Sema3A gastric cancer cell lines. The MTT, clone formation assays were used to observe the Sema3A on gastric cancer cell proliferation. ANNEX- IN V/PI staining by FCM was used to observe the effect of Sema3A on cell apoptosis. Transwell assay was used to ob- serve forced overexpression of Sema3A on the ability of invasion and metastasis of gastric cancer cells in vitro. Re- sults ： Lenti - virus packaging Sema3A sense expression vector transfection into gastric cancer cells MKN - 28M, effec- tively increased the Sema3A gene expression. Compared with the control group, Sema3A transduction to MKN -28M cell decreased cell growth and proliferation ability （ P 〈 0.05 ） , as well as induced cell apoptosis （ P 〈 0.05 ）. Invasive assay in vitro showed that exogenous forced overexpression of Sema3A reduced the ability of invasion and metastasis in the metastasis potential cell lines MKN - 28M （ P 〈 0.05 ）. Conclusion： Successfully constructed effectively overex- pressed Sema3A lenti - virus vector and upregulated the expression of Sema3A in gastric cancer cell lines. Further study made a conclusion that Sema3A decreased the metastasis ability of gastric cancer cell and inhibited cell prolifer- ation and pro - apoptosis which suggested that Sema3A palys an important role in gastric cancer progression.

关 键 词：SEMA3A 胃癌 增殖 转移 

分 类 号：R735.2[医药卫生—肿瘤]