曲面法优化云南野生青头菌多糖的提取工艺  

Study on Extraction Process of Polysaccharide of Russula virescens Optimized by Response Surface Methodology

作　　者：王静[1] 王泽锋[1] 吴娜[1] 陈显兰[1] 刘卫[1] 闵勇[1] 

机构地区：[1]红河学院理学院,蒙自661100

出　　处：《食品工业》2015年第2期181-184,共4页The Food Industry

基　　金：国家自然科学基金(21366011);云南省自然科学基金应用基础研究项目(2013FZ120);云南省红河学院化学学科开放基金(HXY1303)

摘　　要：通过微波辅助提取和乙醇沉法提取青头菌中的多糖,在单因素试验结果的基础上,根据Box-Behnken中心组合试验设计原理,设计3因素3水平试验,利用Design-Expert软件进行响应面分析,建立青头菌多糖的提取的回归方程。经检验该回归方程是合理的可靠的,能够较好的预测青头菌多糖的提取率。结果表明,经响应面优化法确定的最佳提取条件为料液比1∶20(g/m L),提取温度为93℃、提取时间为27 min。经过试验验证,青头菌多糖的提取率为5.69%,与预测值得相对误差为0.35%。The Polysaccharide extraction ofRussula virescens was extracted by means of microwave assisted extraction and the ethanol precipitation method. Based on the single factor experiment results, and according to the experimental design principle of Box-Behnken Center, it designs the experiment of three factors three levels. The software of Design-Expert is adopted to make response surface analysis, thus to build the regression equation of the Polysaccharide extraction ofRussula virescens. By checking, this regression exluation is reasonable and reliable. It can better predict the extraction rate of the Polysaccharide extraction ofRussula virescens. The optimum condition is determined by the method of response surface for the material liquid ratio 1 ： 20 （g/mL）. The extraction temperature is 93 ℃. The extraction time is 3 h. It is verified by experiment that the extraction rate of Polysaccharide extraction ofRussula virescens is 5.69%, and the relative error with prediction is 0.35%.

关 键 词：青头菌 多糖 响应曲面法 模型 预测 验证 

分 类 号：TQ281[化学工程—有机化工]