Fabrication of PEI-Liposome Loaded Fish Gelatin Nanofibers Mats by Green Electrospinning  

作　　者：陈梦霞 林思 王红声 

机构地区：[1]College of Chemistry,Chemical Engineering and Biotechnology,Donghua University

出　　处：《Journal of Donghua University(English Edition)》2014年第5期686-690,共5页东华大学学报（英文版）

基　　金：Natural Science Foundation of Shanghai,China(No.12ZR1400300);"111 Project" Biomedical Textile Materials Science and Technology,China(No.B07024);the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry,China;Open Foundation of State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,China(No.LK1111)

摘　　要：Polyethyleneimine( PEI) and cationic liposomes were widely used for gene delivery and the combination of PEI and liposome was reported to result in a higher efficiency of cell transfection in vitro.In recent years, better transfection was observed for the drug-loaded liposome fixed on the tissue engineering scaffolds via embedding,surface adsorption or covalent grafting thus protected and bound by the scaffolds.In the present study,a novel PEI-liposome loaded fish gelatin composite nanofiber was successfully fabricated by a green electrospinning process.The existence of PEIliposome in the composite nanofibers was determined by Fourier transform infrared( FTIR) spectra, transmission electron microscopy( TEM), and confocal laser scanning microscopy( CLSM).As shown by scanning electron microscopy( SEM),the electrospun composite nanofibers with uniform diameter were smooth and round,and the morphology of the fish gelatin fibers did not change significantly after the incorporation of PEI-liposomes.The transfection results in vitro suggest PEI-liposome loaded fish gelatin material may have a promising application in non-viral gene delivery systems.Polyethyleneimine( PEI) and cationic liposomes were widely used for gene delivery and the combination of PEI and liposome was reported to result in a higher efficiency of cell transfection in vitro.In recent years, better transfection was observed for the drug-loaded liposome fixed on the tissue engineering scaffolds via embedding,surface adsorption or covalent grafting thus protected and bound by the scaffolds.In the present study,a novel PEI-liposome loaded fish gelatin composite nanofiber was successfully fabricated by a green electrospinning process.The existence of PEIliposome in the composite nanofibers was determined by Fourier transform infrared( FTIR) spectra, transmission electron microscopy( TEM), and confocal laser scanning microscopy( CLSM).As shown by scanning electron microscopy( SEM),the electrospun composite nanofibers with uniform diameter were smooth and round,and the morphology of the fish gelatin fibers did not change significantly after the incorporation of PEI-liposomes.The transfection results in vitro suggest PEI-liposome loaded fish gelatin material may have a promising application in non-viral gene delivery systems.

关 键 词：fish GELATIN POLYETHYLENEIMINE ( PEI ) LIPOSOME nanofiber TRANSFECTION 

分 类 号：R318[医药卫生—生物医学工程]