Identification of Cinobufagin and Resibufogenin as Inhibitors of Enterovirus 71 Infection  

作　　者：CHEN Jiawen XU Lin SUN Shiyang ZHANG Huafei MA Tonghui SU Weiheng JIANG Chunlai 

机构地区：[1]National Engineering Laboratory for AIDS Vaccine [2]Key Laboratory for Molecular Enzymology & Engineering, Ministry of Education, School of Life Seiences, Jilin University, Changchun 130012, P. R. China," [3]College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, t~ R. China

出　　处：《Chemical Research in Chinese Universities》2014年第6期953-958,共6页高等学校化学研究（英文版）

摘　　要：In this paper, cinobufagin and resibufogenin were found to inhibit enterovirus 71（EV71） infection in vitro in cell viability and plaque reduction assays. The 50% inhibitory concentrations（lCs0） of einobufagin and resibufoge- nin were （10.94±2.36） and （218±31） nmol/L, respectively, the 50% cytotoxic concentrations（CCs0） of them were （1277±223） and （1385±254） nmol/L, respectively, and the anti-EV71 selectivity index（SI50） of cinobufagin was 116.7, which are promisingly developed into drug. Using a VP1 detection assay and a constructed reporter luciferase, we found that cinobufagin and resibufogenin disrupted the synthesis of EV71 protein. However, neither of them inhibited EV71 RNA replication. Our study suggests that cinobufagin and resibufogenin are the promising candidates that should he fllrther investigated for the treatment of EV71 caused disease.In this paper, cinobufagin and resibufogenin were found to inhibit enterovirus 71（EV71） infection in vitro in cell viability and plaque reduction assays. The 50% inhibitory concentrations（lCs0） of einobufagin and resibufoge- nin were （10.94±2.36） and （218±31） nmol/L, respectively, the 50% cytotoxic concentrations（CCs0） of them were （1277±223） and （1385±254） nmol/L, respectively, and the anti-EV71 selectivity index（SI50） of cinobufagin was 116.7, which are promisingly developed into drug. Using a VP1 detection assay and a constructed reporter luciferase, we found that cinobufagin and resibufogenin disrupted the synthesis of EV71 protein. However, neither of them inhibited EV71 RNA replication. Our study suggests that cinobufagin and resibufogenin are the promising candidates that should he fllrther investigated for the treatment of EV71 caused disease.

关 键 词：Enterovirus 71 Coxsackievirus A16 CINOBUFAGIN RESIBUFOGENIN Chansu 

分 类 号：Q959.530.2[生物学—动物学] TS207.4[轻工技术与工程—食品科学]