谷氨酸棒杆菌串联生产氨基酸和有机酸的发酵工艺  

Production of amino acids and organic acids in series fermentation process by Corynebacterium glutamicum

作　　者：许晟[1] 郝宁[1] 许琳[1] 汤俊[1] 刘兆星[1] 严明[1] 欧阳平凯[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009

出　　处：《南京工业大学学报（自然科学版）》2014年第6期49-53,共5页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：江苏省高校自然科学研究面上项目(13KJB530008);江苏省普通高校研究生科研创新计划(CX10B_179Z)

摘　　要：为进一步提高谷氨酸棒杆菌在工业生产中的利用效率,根据其好氧环境下可发酵生产氨基酸,厌氧条件下可积累有机酸的特性,建立了好氧、厌氧两阶段生产氨基酸及有机酸的串联发酵工艺。研究表明:在好氧条件下谷氨酸棒杆菌以80 g/L葡萄糖为碳源生产L-鸟氨酸15 g/L,糖酸转化率为18.75%;好氧发酵结束后收集菌体转入厌氧发酵工艺,以40 g/L葡萄糖为碳源,发酵液中积累乳酸19.58 g/L、乙酸1.34 g/L、丁二酸18.37 g/L,综合糖酸转化率达到98.88%。整个串联发酵工艺的实现,有效地利用了生物质资源、降低了生产能耗、减小了环境污染,有助于提高整体的经济效益,同时也为后续利用代谢工程进一步改造谷氨酸棒杆菌,并将其应用于更广泛的氨基酸、有机酸联合生产奠定了基础。A new fermentation technique that the combined aerobic fermentation with anaerobic fermentation in series was proposed based on the properties of Corynebacterium glutamicum. They could produce amino acids under the aerobic condition and accumulate organic acids under the anaerobic condition. The fermentation results showed that the ornithine concentration reached 15 g / L from 80 g / L glucose under the aerobic condition while the lactic acid,acetic acid and succinic acid concentration reached 19. 58 g / L,1. 34 g / L and18. 37 g / L from 40 g / L glucose under oxygen deprivation condition,respectively. The yield of amino acids and organic acids from glucose reached 18. 75% and 98. 88%,respectively. Whole series processes improved the overall economic benefits by effectively using the biomass resources,reducing energy consumption and avoiding environmental problems. It also provided the basis for further metabolic engineering of the strain to improve the productivity of amino acids and organic acids.

关 键 词：谷氨酸棒杆菌 氨基酸 有机酸 串联发酵工艺 节能环保 

分 类 号：Q815[生物学—生物工程]