高效液相色谱法同时测定食品中多种添加剂  

Simultaneous Determination of Various Additives in Food by High Performance Liquid Chromatography

作　　者：高文惠[1,2] 庞军[1,2] 高林[1,2] 贾英民[1,2] 

机构地区：[1]河北科技大学生物科学与工程学院,石家庄050000 [2]河北省发酵工程技术研究中心,石家庄050000

出　　处：《中国食品学报》2014年第12期148-153,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家科技支撑计划项目(2013BAD10B03);河北省科技支撑计划项目(14227504D;14236602D-13)

摘　　要：为建立一种应用高效液相色谱法同时测定新红、胭脂红、糖精钠、日落黄、苯甲酸、山梨酸、香兰素、亮蓝、赤藓红9种食品添加剂的分析方法，采用梯度洗脱技术，以C18柱（250mm×4．6mm，5μm）为分离柱，以甲醇－0．02m01／L乙酸铵（pH5．77）－乙腈（乙酸铵溶液与乙腈体积比8．25：1）为流动相，用紫外检测器在波长230nm处检测饮料、酱菜等样品。样品经过处理后直接进样，1次进样分析可在13min内实现基线分离。该方法的线性相关系数范围为0．9979～0．9998，检测限0．025．2．0×g／mL，平均回收率在83．4％～111．4％之间，相对标准偏差（RSD）〈5．5％。该方法灵敏度高，重现性好，操作方便、快速，是检测食品添加剂的有效方法。A method has been developed for simultaneous determination of nine food additives by High performance liquid chromatography（HPLC）, such as C. I. Food Red 10, Ponceau 4R, Saccharin Sodium, C. I. Food Yellow 3, Ben- zoic acid, Sorbic acid, Vanillin, C. I. Food Blue 2 and C.I. Food Red 14. Gradient elution was used in this method. Eclipse ClS column （250 min×4.6 mm,5 μm） was used as separation column. Methanol-0.02 mol/mL ammonium acetate （pH=5.77）-acetonitrile （volume ratio of ammonium acetate and acetonitrile was 8.25：1） was used as mobile phase. Sam- ples such as drinks and pickles were detected at 230 nm by UV detector. The samples could be injected directly after treatment, and the baseline separation was obtained within 13 min. Under these conditions, the linear correlation coeffi- cient range was 0.9979-0.9998, the detection limit was between 0.025 μg/mL and 2.0 μg/mL, the average recovery was between 83.4% and 111.4%, and the relative standard deviation （RSD） was less than 5.5%（n=5）. The method had high sensitivity, good reproducibility, simple operation, rapid determination, and it was an effective method for determining food additives.

关 键 词：食品添加剂 梯度洗脱 同时测定 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] TS207.3[理学—化学]