Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival  

作　　者：Dmitriy Sergeevich Kobyakov Ashot Merudzhanovich Avdalyan Aleksandr Fedorovich Lazarev Elena Leonidovna Lushnikova Lev Moiseevich Nepomnyashchikh 

机构地区：[1]Budget Institution, Kogalym City Hospital [2]Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N.Blokhin Cancer Research Center [3]Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences

出　　处：《Cancer Biology & Medicine》2014年第4期264-269,共6页癌症生物学与医学（英文版）

摘　　要：Objective： To evaluate the relation between argyrophilic nucleolar organizer region （AgNOR）-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer （NSCLC）. Methods： A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 （clone MIB-1） and silver nitrate by immunohistochemical and AgNOR-staining methods. Results： The AgNOR area in MIB-l-positive cells of NSCLC is related to clinicopathological parameters under the TNM （tumor, node, and metastasis） system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological （AgNOR area in MIB-l-positive cells）, and clinicopathological （greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation） parameters are independent prognostic factors of NSCLC.Conclusion： The AgNOR area in MIB- 1-positive cells is related to clinicopathological parameters and survival in NSCLC.Objective:To evaluate the relation between argyrophilic nucleolar organizer region(Ag NOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer(NSCLC).Methods:A total of 207 surgical specimens diagnosed as NSCLC were included in this study.Double-staining procedures were performed using antigen Ki-67(clone MIB-1)and silver nitrate by immunohistochemical and Ag NOR-staining methods.Results:The Ag NOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM(tumor,node,and metastasis)system.The survival of patients with small Ag NOR area in MIB-1-positive cells is better than that of patients with large Ag NOR area.Molecular,biological(Ag NOR area in MIB-1-positive cells),and clinicopathological(greatest tumor dimension,metastases to regional lymph nodes,histology,and differentiation)parameters are independent prognostic factors of NSCLC.Conclusion:The Ag NOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC.

关 键 词：Argyrophilic nucleolar organizer region （AgNOR） MIB-1 SURVIVAL non-small cell lung cancer （NSCLC） 

分 类 号：R734.2[医药卫生—肿瘤]