量子点探针应用于粮油中黄曲霉毒素免疫检测的研究  

Quantum dots label in immunoassay detection of aflatoxins in cereals and oils

作　　者：许琳[1,2,3] 张兆威[1,2,3,4] 李培武[1,2,3,4,5] 张奇[1,2,3,4] 张文[1,3,4,5] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [3]农业部生物毒素检测重点实验室,湖北武汉430062 [4]农业部油料产品质量安全风险评估实验室,湖北武汉430062 [5]农业部油料及制品质量监督检验测试中心,湖北武汉430062

出　　处：《中国油料作物学报》2015年第1期119-123,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31101299);国家科技支撑计划(2012BAB19B09);公益性行业(农业)科研专项(201203094)

摘　　要：为改良检测技术,拓宽量子点探针在农产品安全领域的应用范围,制备了用于粮油中黄曲霉毒素检测的2种量子点探针,即碳量子点(CQD)探针和石墨烯量子点(GQD)荧光免疫探针,研究2种探针应用于黄曲霉毒素检测中的可行性。采用绿色合成方法,发现以0.375g/m L柠檬酸水溶液中获得的石墨烯量子点荧光强度最强。利用1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC·HCL)和N-羟基琥珀酰亚胺(NHS),将碳量子点和GQD与抗黄曲霉毒素的单克隆抗体1C11进行共价偶联,发现偶联前后量子点的荧光光谱变化趋势一致,而且偶联后均保持了较好的荧光特性,因此均可作为黄曲霉毒素免疫检测的探针,且与抗体偶联后的碳量子点的荧光特性优于石墨烯量子点。To develop quantum dot application on food safety detection,CQDs( carbon quantum dots) and GQDs( graphene quantum dots) were evaluated as the fluorescence labels in aflatoxins detection in cereals and oils. Using environment friendly method,GQDs were synthesized directly in citric acid solution,and showed the strongest fluorescence intensity under 0. 375 g / m L citric acid. Then the GQDs and CQDs were covalent coupled with monoclonal antibody 1C11 by 1- ethyl- 3-( 3- dimethylaminoproyl) carbodiimide hydrochloride( EDC) and N- hydroxyl- sulfosuccinimide sodium salt( NHS). Results showed that both quantum dots had strong fluorescence intensities,which could be served as fluorescence labels in aflatoxin immunoassay. CQDs fluorescence property was stronger than that of GQDs after coupled with antibody.

关 键 词：碳量子点 石墨烯量子点 荧光免疫探针 黄曲霉毒素 

分 类 号：S379.7[农业科学—农产品加工]