反胶束固定化醇脱氢酶的制备及应用研究  

Preparation and Application of Immobilized Alcohol Dehydrogenase with Reversed Micelles

作　　者：柳畅先[1] 何进星[1] 

机构地区：[1]中南民族大学化学与材料科学学院,分析化学国家民委重点实验室,湖北武汉430074

出　　处：《分析科学学报》2015年第1期119-122,共4页Journal of Analytical Science

摘　　要：研究十六烷基三甲基溴化铵(CTAB)-辛烷-己醇反胶束体系固定化醇脱氢酶(ADH)的制备及应用。考察了含水量、CTAB和己醇用量对于ADH固定化的影响。对游离酶和固定化酶的催化动力学性质研究表明:酶促反应的最适pH值分别为8.2和8.8,最适温度分别为31℃和20℃,米氏常数分别为12mmol/L和7mmol/L。30℃时,游离酶存放150min后失活90%,固定化酶失活50%,表明反胶束固定化ADH有较好的热稳定性。应用此体系测定了试样中乙醇的含量。In this paper,the preparation and application of immobilized alcohol dehydrogenase（ADH）with cetyltrimethyl ammonium bromide（CTAB）-octane-hexanol reversed micelles system are studied.The effects of water content,the concentration of CTAB and hexanol on the immobilization of ADH were investigated.The results from the kinetic properies of the free and immobilized enzyme indicate that optimum pH of the enzymatic reaction were 8.2and 8.8,the optimum temperature were 31℃and 20℃,and the michaelis constant were 12 mmol/L and 7 mmol/L,respectively.The free and immobilized enzyme lost 90% and 50% of their activities after being kept at 30℃for 150 min respectively,showing that the immobilized ADH in reversed micelles has better thermal stability.The system has been used to determine the concentration of alcohol in samples.

关 键 词：反胶束 醇脱氢酶 固定化 酶法测定 

分 类 号：O657.3[理学—分析化学]