新疆阿魏抗氧化活性部位研究  

Study on Anti-oxidant Activity Parts of Ferula sinkiangensis K. M. Shen

作　　者：张海英[1,2] 周龙龙[1] 姜林[2] 周铭心[1] 

机构地区：[1]新疆医科大学,新疆乌鲁木齐830011 [2]新疆医科大学附属中医医院,新疆乌鲁木齐830000

出　　处：《中国中医药信息杂志》2015年第3期80-82,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81260625)

摘　　要：目的建立高通量的药物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力的检测方法,用于新疆阿魏抗氧化活性部位的初步筛选。方法以抗坏血酸的DPPH自由基清除率为阳性对照,并以半抑制浓度(IC50)作为评价各供试品清除DPPH自由基能力的指标,评价和筛选新疆阿魏各极性部位(石油醚部、乙酸乙酯部、甲醇部、水部)的抗氧化能力。结果新疆阿魏不同极性部位对DPPH自由基清除的IC50分别为石油醚部3252.22μg/m L、乙酸乙酯部36.22μg/m L、甲醇部32.22μg/m L、水部2643.38μg/m L,抗坏血酸为27.16μg/m L。结论乙酸乙酯部、甲醇部为新疆阿魏清除DPPH自由基的有效抗氧化活性部位。本研究建立了简便、灵敏的药物对DPPH自由基清除测定方法,其高通量的快速检测可为其他抗氧化药物的筛选提供参考。Objective To establish a high-throughput detection of medicine on DPPH free radical scavenging activity;To analyze and preliminarily screen the anti-oxidant activity part of Ferula sinkiangensis. Methods Taking DPPH free radical scavenging rate of ascorbic acid as positive control, and IC50 as the evaluating criterion of DPPH free radical scavenging capability, oxidation resistance of different polar parts （petroleum ether part, ethyl acetate part, methyl alcohol part, and water part） of Ferula sinkiangensis was evaluated and screened. Results Different polarity parts of Ferula sinkiangensis DPPH radical scavenging of IC50 were：petroleum ether part 3252.22 μg/mL, ethyl acetate part 36.22 μg/mL, methyl alcohol part 32.22 μg/mL, water part 2643.38 μg/mL, and positive control group ascorbic acid 27.16 μg/mL. Conclusion The ethyl acetate and methyl alcohol parts of Ferula sinkiangensis were effective anti-oxidant activity site to eliminate DPPH free radicals. In this study, a simple, sensitive, and high-throughput detection method of DPPH radical scavenging assay was established to provide reference for the anti-oxidation medicine detection screening.

关 键 词：新疆阿魏 1 1-二苯基-2-三硝基苯肼自由基 抗氧化活性 抗坏血酸 

分 类 号：R284.1[医药卫生—中药学]