壳聚糖盐酸盐对几种水产致病菌的抑菌性研究  

Inhibitory effect of chitosan hydrochloride on several aquatic pathogens

作　　者：孙玉英[1] 张继泉[2] 

机构地区：[1]江苏省海洋资源开发研究院淮海工学院,江苏连云港222005 [2]中国科学院海洋研究所实验海洋生物学重点实验室,山东青岛266071

出　　处：《中国酿造》2015年第1期47-49,共3页China Brewing

基　　金：国家自然科学基金项目(41306165);碳谷-江苏海资院海洋先进材料联合研究中心项目(TG-201402)

摘　　要：目前,对虾疾病仍然是困扰对虾养殖业的一大难题,由弧菌属(Vibrio)细菌引起的弧菌病(Vibriosis)是其中危害最大的细菌性疾病,给水产养殖业造成了严重的经济损失。本实验以副溶血弧菌、哈维氏弧菌和鳗弧菌为供试菌种,采用滤纸片扩散法对壳聚糖盐酸盐进行抑菌实验研究。在此基础上进一步采用96孔板法测定了壳聚糖盐酸盐对副溶血弧菌、哈维氏弧菌和鳗弧菌的最小抑菌浓度(MIC),结果表明:壳聚糖盐酸盐对3种供试菌株均具有抑菌作用,最小抑菌质量浓度因供试菌的不同而不同,副溶血弧菌为2.5 mg/m L,哈维氏弧菌和鳗弧菌为3.25 mg/m L。At present,prawn disease is still a problem plaguing shrimp farming.The Vibriosis caused by Vibrio is one of the most harmful bacterial diseases which causes serious economic losses.The inhibitory effect of chitosan hydrochloride on several aquatic pathogens,including Vibrio parahaemolyticus,V.harveyi and V.anguillarum was analyzed using filter paper diffusion method.The minimum inhibitory concentrations （MIC） of chitosan hydrochloride against the three test pathogens were measured by a further 96-well plate assay.The results showed that chitosan hydrochloride could inhibit the growth of the three test pathogens; however,MIC was different with different pathogen.The MIC of V.parahaemolyticus was 2.5 mg/ml,and V.harvey and V.anguillarum was 3.25 mg/ml.

关 键 词：壳聚糖盐酸盐 滤纸片扩散法 96孔板法 抑菌作用 

分 类 号：S945.1[农业科学—水产养殖]