富含谷维素稻米油通过调节单胺类神经递质改善睡眠剥夺小鼠睡眠状况（英文）  

Effects of Rice Bran Oil Rich in Oryzanol on PentobarbitalInduced Sleeping Behaviors in Partial Sleep-Deprived Mice Through Modulation of Monoamines

作　　者：杨阳[1,2] 陈贵[1,3] 朱翠红[1] 罗晓林[1] 黄碧霞[1] 朱惠莲[1] 

机构地区：[1]中山大学公共卫生学院营养学系,广州510080 [2]河南省人民医院营养科,郑州450001 [3]佛山市顺德区卫生监督所,佛山528300

出　　处：《营养学报》2014年第6期577-583,I0005,共8页Acta Nutrimenta Sinica

摘　　要：目的谷维素是一种富含于稻米油中具有生物活性的天然植物化学物,被用于治疗自主神经功能紊乱和应激,但很少有关于谷维素在缓解疲劳及改善睡眠方面作用的报道。本研究探讨富含谷维素稻米油对部分睡眠剥夺小鼠睡眠状况及自主活动的影响。方法将60只小鼠随机分成对照组、部分睡眠剥夺(PSD)模型组、低浓度谷维素组(PSD-l OZ)、中浓度谷维素组(PSD-m OZ)和高浓度谷维素组(PSD-h OZ),每组12只。对照组和PSD模型组喂食对照饲料(含8%大豆油),PSD-l OZ、PSD-m OZ和PSD-h OZ组喂食含稻米油(8%)饲料,稻米油中谷维素含量分别为为3000、7000、15000 mg/kg。以相应饲料喂食25 d后对除对照组之外的小鼠进行部分睡眠剥夺。睡眠剥夺结束后进行自主活动测定和戊巴比妥钠诱导睡眠实验。采用HPLC-ESI-MS/MS法测定小鼠大脑组织中NE、DA和5-HT含量,睡眠剥夺后立即测定自主活动。结果 PSD模型组小鼠自主活动次数明显低于对照组(P<0.05),PSD-m OZ组和PSD-h OZ组小鼠自主活动次数均大于PSD模型组(均P<0.05);PSD-m OZ组和PSD-h OZ组小鼠的戊巴比妥钠睡眠潜伏期显著短于PSD模型组(both P<0.05);简单线性回归分析结果显示稻米油中谷维素含量越高,小鼠睡眠时间越长(P=0.05);PSD模型组小鼠大脑NE、DA和5-HT含量低于对照组(均P<0.05),PSD-h OZ组小鼠大脑NE、DA和5-HT含量显著高于PSD模型组(均P<0.05)。结论富含谷维素稻米油可以缓解睡眠剥夺所致的疲劳并且可以改善睡眠,其机制可能与其能有效抵抗睡眠剥夺所致单胺类神经递质的下降有关。Objective To investigate whether rice bran oil rich in oryzanol has effects on pentobarbital-induced sleeping behaviors and locomotor activities in partial sleep-deprived mice.Methods Sixty mice were randomly divided into five groups （12 for each）. Control and partial sleep deprivation （PSD） model groups were fed basic rodent chow with 8% soy-bean oil （oryzanol free）. PSD-low oryzanol （PSD-IOZ）, PSD-medium oryzanol （PSD-mOZ） and PSD-high oryzanol （PSD-hOZ） groups were fed basic rodent chow with 8% rice bran oil, containing 3000, 7000 and 15000 mg/kg of oryzanol, respectively for 25 d. The locomotor activities after PSD and the latency and duration of sleep induced by pentobarbital were determined. The contents of monoamines, as norepinephrine （NE）, dopamine （DA） and serotonin （5-HT） were measured by HPLC-ESI-MS/MS. Results Immediately after PSD, the locomotor activities in PSD model group were decreased compared with the control group （P 〈 0.05）. The PSD-mOZ and PSD-hOZ groups had higher locomotor activities than the PSD model group （both P 〈 0.05）. The latency of sleep in the PSD-mOZ group and PSD-hOZ group were both decreased when compared with the PSD model group （both P 〈 0.05）. Linear regression showed that the duration of sleep time increased in dose dependent manner with the contents of oryzanol in rice bran oil （P = 0.05）. The PSD model group had lower level of NE, DA and 5-HT when compared with the control group （all P〈0.05）. Supplementation of high contents of oryzanol in rice bran oil may prevent the decline in monoamines （NE, DA, 5-HT） induced by PSD （all P〈0.05）. Conclusion Rice bran oil rich in oryzanol may alleviate fatigue and improve sleep in mice with PSD through modulation of monoamines （NE, DA, 5-HT）.

关 键 词：部分睡眠剥夺 稻米油 谷维素 自主活动 睡眠状况 单胺类(NE DA 5-HT) 

分 类 号：R151[医药卫生—营养与食品卫生学]