荧光法测定药品中维生素B_2含量的综合型实验设计  

Design and Exploration of Comprehensive Experiment for Fluorescence Determination of Vitamin B_2 in Tablets

作　　者：程春英[1] 尹学博[1] 

机构地区：[1]南开大学化学学院化学实验教学中心和分析科学研究中心,天津300071

出　　处：《实验室研究与探索》2014年第12期159-162,共4页Research and Exploration In Laboratory

基　　金：国家自然科学基金(21375064);高校博士点基金项目(20130031110016);973项目(2011CB707703)

摘　　要：将本科实验转型为研究性、综合型实验对于提高学生的实验技能、发散思维以及发现问题和解决问题的能力发挥着重要的作用。讨论了将荧光分光光度法测维生素B2的含量实验调整为研究性、综合型实验的内容和过程,包括学生的课前预习以了解维生素B2结构、性质等;通过考察p H对荧光性质的影响确定最优的检测p H条件;与紫外分光光度法结合,考察光照产物吸收光谱和荧光光谱的变化,提出维生素B2光分解的初步机理;分析了实际药品中维生素B2测定中可能存在的干扰。以此为基础建立了药品中维生素B2测定的荧光光谱法。The cultivation of undergraduate＇s experimental skill and comprehensive quality is becoming an important goal of higher education,especially,the experimental education. The design and development of research-oriented and comprehensive experiments play an important role for this purpose. The fluorescence method for the determination of vitamin B2 is designed as research experiment. The method can find the structure and properties of vitamin B2 prior to the class. The optimal condition is obtained through the investigation of the effect of p H on the fluorescence of vitamin B2. The investigation of its absorption and fluorescence spectra after being submitted to sunshine is used to learn the preliminary mechanism of photolysis of vitamin B2. The interference on the determination of vitamin B2 is also tested.The fluorescence method for the determination of vitamin B2 in tablet is therefore built.

关 键 词：研究性实验 本科实验 维生素B2测定 

分 类 号：O6-3[理学—化学]