经支气管镜超声引导针吸活检术标本确诊肺腺癌患者表皮生长因子受体突变分析  

Analysis of Epidermal Growth Factor Receptor(EGFR) Gene Mutations in Pathologically Confirmed Lung Adenocarcinoma by Endobronchial Ultrasound-guided Transbronchial Needle Aspiration

作　　者：陈苏峰[1,2] 张亚伟[1,2] 胡鸿[1,2] 张杰 孙艺华[1,2] 王龙富[2,3] 陈颖[2,3] 王彦丽[2,3] 相加庆[1,2] 陈海泉[1,2] 

机构地区：[1]复旦大学附属肿瘤医院胸外科 [2]复旦大学上海医学院肿瘤学系,上海200032 [3]复旦大学附属肿瘤医院病理科

出　　处：《中国胸心血管外科临床杂志》2015年第1期44-48,共5页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：上海市卫生局课题(2011032);上海卫生系统重要疾病联合攻关重大项目(2013ZYJB0301)~~

摘　　要：目的分析经支气管镜超声引导针吸活检术（endobronchial ultrasound-guided transbronchial needle aspiration,EBUS-TBNA）获取标本确诊肺腺癌患者表皮生长因子受体（epidermal growth factor receptor,EGFR）基因突变状况。方法回顾性分析复旦大学附属肿瘤医院胸外科2009年4月至2013年9月连续964例EBUS-TBNA术患者的临床资料,分析经细胞形态学和免疫组织化学染色检查确诊为肺腺癌的77例患者的EGFR基因突变情况。其中男48例、女29例,中位年龄61（33~78）岁,有吸烟史患者43例。结果 77例肺腺癌患者均经免疫酶标检测确认,全组共有EGFR基因突变患者31例,突变率为40.26%。EGFR基因突变患者性别差异无统计学意义（P=0.088）;与吸烟的患者相比较,不吸烟的患者EGFR基因突变率较高,差异有统计学意义（P=0.032）。结论 EBUS-TBNA术获取的标本可以进行EGFR基因突变检测并获得准确的基因突变信息,不吸烟的患者EGFR基因突变比率较高。Objective To analyse epidermal growth factor receptor（EGFR） gene mutations in pathologically confirmed lung adenocarcinoma（LAC） samples obtained by endobronchial ultrasound-guided transbronchial needle aspiration（EBUS-TBNA）. Methods Clinical data of 964 consecutive patients who underwent EBUS-TBNA in Department of Thoracic Surgery, Fudan University Shanghai Cancer Center from April 2009 to September 2013 were retrospectively reviewed. EGFR gene mutations in 77 LAC patients who were comfirmed by cell morphology and immunohistochemistry were analyzed. There were 48 males and 29 females with their median age of 61（range 33-78）years, and 43 patients were smokers. Results All the 77 LAC patients were confirmed by immunohistochemistry. Among them, 31 patients（40.26%） were found to have EGFR gene mutations. There was no statistical difference in EGFR gene mutations between male and female patients（P=0.088）. Mutation rate of EGFR genes of non-smokers was significantly higher than that of smokers（P=0.032）. Conclusion Samples obtained by EBUS-TBNA can be used for EGFR gene mutations analysis. The mutation rate of EGFR genes of non-smokers is higher than that of smokers.

关 键 词：肺肿瘤 支气管内超声 经支气管针吸活检 基因 突变 

分 类 号：R734.2[医药卫生—肿瘤]